High throughput flow cytometer operation with data quality assessment and control
First Claim
1. A flow system for reliable multiparameter data acquisition and particle sorting and enumeration, the system comprising:
- a fluidics system comprising a moving fluid column within which particles of a sample move along a common sample path;
a detection system for collecting one or more signals from each particle as it passes one or more detection stations along the common sample path, each signal being assigned a signal value to form a multiparameter data point for each particle, the detection system collecting in a succession of different time intervals such multiparameter data points in one or more predetermined subsets to form a data profile for each of such time intervals;
a control system operationally associated with the fluidics system and detection system, the control system determining a data profile characteristic of the current data profile and comparing the data profile characteristic either with that of a predetermined prior data profile or with a predetermined limit, and whenever such data profile characteristic exceeds the predetermined limit or differs from the data profile characteristic of the predetermined prior data profile, the control system actuating a corrective action.
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Abstract
The invention provides a flow system and method for reliable multiparameter data acquisition and particle sorting. In accordance with the invention, a flow system assesses changes in the pattern of data collected in successive time intervals and actuates one or more corrective actions whenever the changes exceed predetermined limits. The present invention overcomes problems associated with collecting data and sorting and enumerating particles in flow cytometry systems that operate for prolonged periods or that must accommodate samples that vary widely in quality.
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Citations
23 Claims
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1. A flow system for reliable multiparameter data acquisition and particle sorting and enumeration, the system comprising:
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a fluidics system comprising a moving fluid column within which particles of a sample move along a common sample path; a detection system for collecting one or more signals from each particle as it passes one or more detection stations along the common sample path, each signal being assigned a signal value to form a multiparameter data point for each particle, the detection system collecting in a succession of different time intervals such multiparameter data points in one or more predetermined subsets to form a data profile for each of such time intervals; a control system operationally associated with the fluidics system and detection system, the control system determining a data profile characteristic of the current data profile and comparing the data profile characteristic either with that of a predetermined prior data profile or with a predetermined limit, and whenever such data profile characteristic exceeds the predetermined limit or differs from the data profile characteristic of the predetermined prior data profile, the control system actuating a corrective action. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. A flow system for reliable multiparameter data acquisition and particle sorting and enumeration, the system comprising:
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a fluidics system comprising a sample tube for inserting sample into a moving fluid column as a sample stream within which particles of a sample move along a common sample path, the sample stream containing free fluorescence; a detection system for collecting one or more signals from each particle as it passes one or more detection stations along the common sample path, each signal being assigned a signal value to form a multiparameter data point for each particle, each multiparameter data point including a component comprising a signal value for free fluorescence in the sample stream adjacent to such particle, and the detection system collecting in a succession of different time intervals such multiparameter data points in one or more predetermined subsets to form a data profile for each of such time intervals; a control system operationally associated with the fluidics system and detection system, the control system determining a data profile characteristic of the current data profile and comparing the data profile characteristic either with that of a predetermined prior data profile or with a predetermined limit, and whenever such data profile characteristic exceeds the predetermined limit or differs from the data profile characteristic of the predetermined prior data profile, the control system actuating a corrective action. - View Dependent Claims (18)
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19. A method for reliable data acquisition and sorting in a flow system, the method comprising the steps of:
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providing a moving fluid column within which particles of a sample move along a common sample path; collecting a plurality of signals from each particle as it passes one or more detection stations along the common sample path, each signal of the plurality being assigned a signal value to form a multiparameter data point for each particle, the detection system enumerating such multiparameter data points in a plurality of predetermined subsets during successive time intervals to form a data profile having a data profile characteristic for each of such time intervals; actuating one or more corrective actions whenever the data profile characteristic of a time interval exceed a predetermined limit. - View Dependent Claims (20, 21, 22, 23)
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Specification