SELF PROCESSING PLANTS AND PLANT PARTS
First Claim
1. An expression cassette comprising a polynucleotide encoding an α
- -amylase operably linked to a promoter and a signal sequence, wherein the signal sequence directs the α
-amylase to a specific compartment allowing the α
-amylase to be localised in a manner that it will not come into contact with its substrate.
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Accused Products
Abstract
The invention provides polynucleotides, preferably synthetic polynucleotides, which encode processing enzymes that are optimized for expression in plants. The polynucleotides encode mesophilic, thermophilic, or hyperthermophilic processing enzymes, which are activated under suitable activating conditions to act upon the desired substrate. Also provided are “self-processing” transgenic plants, and plant parts, e.g., grain, which express one or more of these enzymes and have an altered composition that facilitates plant and grain processing. Methods for making and using these plants, e.g., to produce food products having improved taste and to produce fermentable substrates for the production of ethanol and fermented beverages are also provided.
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Citations
23 Claims
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1. An expression cassette comprising a polynucleotide encoding an α
- -amylase operably linked to a promoter and a signal sequence, wherein the signal sequence directs the α
-amylase to a specific compartment allowing the α
-amylase to be localised in a manner that it will not come into contact with its substrate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
- -amylase operably linked to a promoter and a signal sequence, wherein the signal sequence directs the α
Specification