APPARATUS AND METHOD FOR PERFORMING LIGAND BINDING ASSAYS ON MICROARRAYS IN MULTIWELL PLATES
First Claim
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1. An apparatus, comprising:
- a plate having therein a plurality of wells, each of the wells having a planar bottom surface and being adapted to receive a sample for analysis, wherein the plate has a transparent planar underside; and
an array of prisms affixed to the underside, each prism of the array being in registry with a corresponding one of the plurality of wells.
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Abstract
Ellipsometry systems for imaging binding events between analytes in a sample and an array of ligands in an evanescent field generated by a beam of light reflected from the plane of the ligands is adapted to a multiwell plate structure in common use. In one example, a film of prism arrays is affixed to the underside of the plate with each prism array located in registry with a well and ligand arrays being immobilized on the (planar) bottom surface of the wells. The prism array may be formed in a film and juxtaposed with the bottom surface of the plate or a prism array can be made integral with the plate bottom of a multiwell plate.
99 Citations
17 Claims
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1. An apparatus, comprising:
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a plate having therein a plurality of wells, each of the wells having a planar bottom surface and being adapted to receive a sample for analysis, wherein the plate has a transparent planar underside; and an array of prisms affixed to the underside, each prism of the array being in registry with a corresponding one of the plurality of wells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A system, comprising:
a plate having therein a plurality of wells, each of the wells having a planar bottom surface and being adapted to receive a sample for analysis, wherein the plate has a transparent planar underside, and an array of prisms affixed to the underside, each prism of the array being in registry with a corresponding one of the plurality of wells; and
a source of a beam of light adapted to direct the beam to a selected area of the array in a manner to achieve total internal reflection and to generate an evanescent field in the plane of the associated array of immobilized ligands.- View Dependent Claims (11, 12, 13, 14, 15)
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16. A method of detecting binding events at an array of ligands immobilized on planar bottom surfaces of wells of a multiwell plate, the method comprising:
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transmitting a beam of polarized light to a prism film affixed to a bottom surface of the plate, the prism film being of a material and geometry to permit the beam of polarized light incident thereto to be totally internally reflected in a manner to generate an evanescent field in the plane of the ligand array at which the beam is directed; and obtaining an image of intensity variations in the reflected beam. - View Dependent Claims (17)
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Specification