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Genomic Sequencing

  • US 20090298064A1
  • Filed: 05/29/2008
  • Published: 12/03/2009
  • Est. Priority Date: 05/29/2008
  • Status: Abandoned Application
First Claim
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1. A method for genome sequencing, the method comprising:

  • as a random function, selecting a subset of fragments of a target genome;

    replicating each fragment into clones;

    ordering the clones into clone contigs based on sets of overlapping clones;

    determining potential read overlaps from clone read data and validating base pairs of each read;

    reading local assemblies of contigs from regions smaller than a clone length and assembling the local assemblies into read sets;

    combining the assembled read sets into clone-sized regions; and

    assembling the clone-sized regions into clone contigs.

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