GROWTH FACTORS FOR PRODUCTION OF DEFINITIVE ENDODERM
First Claim
Patent Images
1. A method for generating definitive endoderm, said method comprising:
- providing an in vitro cell population comprising pluripotent human cells with a factor selected from the group consisting of GDF8, GDF11 and a combination of GDF8 and GDF11 in an amount sufficient to induce pluripotent human cells of said cell population to differentiate into definitive endoderm cells.
0 Assignments
0 Petitions
Accused Products
Abstract
Disclosed herein are methods for generating endoderm lineage type cells derived from human pluripotent cells, such as human embryonic stem cells, by using various agents including, but not limited to, GDF8, GDF11 and GSK-3beta inhibitors. Also disclosed herein are endoderm lineage cell populations or compositions, such as populations or compositions comprising definitive endoderm and/or other definitive endoderm-derived cell types.
131 Citations
22 Claims
-
1. A method for generating definitive endoderm, said method comprising:
providing an in vitro cell population comprising pluripotent human cells with a factor selected from the group consisting of GDF8, GDF11 and a combination of GDF8 and GDF11 in an amount sufficient to induce pluripotent human cells of said cell population to differentiate into definitive endoderm cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
Specification