Lateral Flow and Flow-through Bioassay Devices Based On Patterned Porous Media, Methods of Making Same, and Methods of Using Same
First Claim
Patent Images
1. An assay device, comprising:
- a porous, hydrophilic medium;
a fluid impervious barrier comprising polymerized photoresist, the barrier substantially permeating the thickness of the porous, hydrophilic medium and defining a boundary of an assay region within the porous, hydrophilic medium; and
an assay reagent in the assay region.
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Abstract
Embodiments of the invention provide lateral flow and flow-through bioassay devices based on patterned porous media, methods of making same, and methods of using same. Under one aspect, an assay device includes a porous, hydrophilic medium; a fluid impervious barrier comprising polymerized photoresist, the barrier substantially permeating the thickness of the porous, hydrophilic medium and defining a boundary of an assay region within the porous, hydrophilic medium; and an assay reagent in the assay region.
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Citations
86 Claims
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1. An assay device, comprising:
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a porous, hydrophilic medium; a fluid impervious barrier comprising polymerized photoresist, the barrier substantially permeating the thickness of the porous, hydrophilic medium and defining a boundary of an assay region within the porous, hydrophilic medium; and an assay reagent in the assay region. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
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26. An assay device, comprising:
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a porous, hydrophilic medium; a fluid impervious barrier substantially permeating the thickness of the porous, hydrophilic medium and having a width between about 1 mm and about 100 μ
m, the barrier completely defining a boundary of an assay region within the porous, hydrophilic medium; andan assay reagent in the assay region. - View Dependent Claims (27, 28, 29, 30, 31, 32, 33)
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34. An assay device, comprising:
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a porous, hydrophilic medium; a fluid impervious barrier substantially permeating the thickness of the porous, hydrophilic medium and having a length and a width that varies by less than about 10% along the length of the barrier, the barrier defining a boundary of an assay region within the porous, hydrophilic medium, and an assay reagent in the assay region. - View Dependent Claims (35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45)
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46. A method of making a device, the method comprising:
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saturating a porous, hydrophilic medium with photoresist; exposing the saturated medium to a pre-determined pattern of light; removing the photoresist from a region of the medium based on the pre-determined pattern of light to define a barrier of residual photoresist that forms a boundary of the region, wherein the pre-determined pattern of light is selected so that the barrier defines an assay region in the region; and providing an assay reagent in the assay region. - View Dependent Claims (47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69)
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70. A method of making a device, the method comprising:
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coating a stamp of pre-determined pattern with a curable polymer; pressing the coated stamp onto a porous, hydrophilic medium, the medium having a thickness and the curable polymer substantially permeating the medium through its thickness in accordance with the pre-determined pattern; curing the curable polymer so as to form a fluid impervious barrier embedded in the medium, the fluid impervious barrier defining an assay region in the medium; and providing a reagent in the assay region. - View Dependent Claims (71, 72)
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73. A method of performing an assay to determine the presence of an analyte in a liquid sample, the method comprising:
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depositing the liquid sample on an assay device, the assay device comprising a porous, hydrophilic medium, a fluid impervious barrier comprising polymerized photoresist, the barrier substantially permeating the thickness of the porous, hydrophilic medium and defining a boundary of an assay region within the porous, hydrophilic medium, and an assay reagent in the assay region, the assay reagent selected to provide a visible response to the presence of the analyte; obtaining an image of the assay region; and determining the presence of the analyte in the liquid based on the image of the assay region. - View Dependent Claims (74, 75, 76, 77)
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78. A three-dimensional microfluidic device comprising:
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a plurality of patterned porous, hydrophilic layers, wherein each said patterned porous, hydrophilic layer comprises a fluid-impermeable barrier that substantially permeates the thickness of said patterned porous, hydrophilic layer and defines a boundary of one or more hydrophilic regions within each said patterned porous, hydrophilic layer; and a fluid-impermeable layer disposed between every two adjacent said patterned porous, hydrophilic layers, wherein said fluid-impermeable layer comprises one or more openings, said openings in flow communication with at least one hydrophilic region in at least one patterned porous, hydrophobic layer. - View Dependent Claims (79, 80, 81, 82, 83, 84, 85, 86)
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Specification