System for Screening Particles
First Claim
1. A method of screening particles in vivo, the method comprising steps of:
- providing a library comprising a plurality of particle populations that vary in at least one particle characteristic, wherein each population comprises a plurality of particles having substantially the same characteristics;
administering the library of particles to an animal under conditions in which the particles can migrate to a targeted tissue or cells; and
recovering a first plurality of particles that have migrated to the targeted tissue or cells.
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Accused Products
Abstract
Screening of a library of particles in vivo and/or in vitro using Polyplex Iterative Combinatorial Optimization (PICO) allows for the design of particles for targeting a specific organ, tissue (e.g., cancer), or cell. Particles may, for example, include different targeting agents (e.g., aptamers or plurality of aptamers) on their surfaces, and the aptamer or aptamers may be evolved to provide better targeting of the particles. Libraries of particles are enriched in characteristics of particles that have been found to migrate to a tissue of interest, be taken up by cells, etc. The process may be repeated to engineer particles of a desired specificity or biological function.
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Citations
134 Claims
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1. A method of screening particles in vivo, the method comprising steps of:
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providing a library comprising a plurality of particle populations that vary in at least one particle characteristic, wherein each population comprises a plurality of particles having substantially the same characteristics; administering the library of particles to an animal under conditions in which the particles can migrate to a targeted tissue or cells; and recovering a first plurality of particles that have migrated to the targeted tissue or cells. - View Dependent Claims (2, 3, 4, 5, 7, 11, 12, 13, 14, 17, 18, 21, 22, 23, 24, 46)
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6. (canceled)
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8-10. -10. (canceled)
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15-16. -16. (canceled)
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19-20. -20. (canceled)
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25-45. -45. (canceled)
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47. A method of screening particles, comprising:
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providing a library comprising a plurality of particle populations, wherein each population of particles is identified by an analytical signature provided by at least one label, and wherein each population comprises a plurality of particles having substantially the same analytical signature and same characteristics; incubating a population of cells with the library of particles for a predetermined period of time under conditions such that the cells can take up the particles, wherein the particles optionally include a substance that is necessary for the growth or survival of the cells; recovering particles from the living cells; and characterizing the recovered particles. - View Dependent Claims (60)
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48-59. -59. (canceled)
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61-65. -65. (canceled)
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66. A method of screening particles, the method comprising steps of:
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providing a first library comprising a plurality of particle populations that vary by at least one particle characteristic, wherein each population comprises a plurality of particles having substantially the same characteristics, and wherein each of the particle populations includes a targeting agent conjugated to the surface of the particles; administering the first library to an animal; identifying the targeting agent conjugated to those particle populations from the first library that preferentially accumulate in a targeted tissue or cell type; providing a second library comprising a plurality of particle populations that vary in at least one particle characteristic selected from composition, size, surface chemistry, targeting agent, and density of a targeting agent at the surface of the particle, wherein each population comprises a plurality of particles having substantially the same characteristics; and identifying the at least one particle characteristic of those particle populations from the second library that preferentially accumulate in a target tissue or cell type.
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67-132. -132. (canceled)
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133. A method of screening particles, the method comprising steps of:
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providing a library of particles, wherein each particle has a different collection of aptamers conjugated to its surface; administering the library to an animal under conditions suitable for at least one of the particles to migrate to a targeted tissue or cells; and recovering at least one particle from the targeted tissue or cells.
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134-135. -135. (canceled)
Specification