COMPOUNDS AND METHODS FOR LABELING OLIGONUCLEOTIDES

• US 20090299041A1
• Filed: 08/04/2009
• Published: 12/03/2009
• Est. Priority Date: 05/20/2005
• Status: Active Grant

• Current Assignee: Integrated DNA Technologies (Danaher Corporation)
• Original Assignee: Integrated DNA Technologies (Danaher Corporation)
• Inventors: Laikhter, Andrei, Yong, Yawfui, Walder, Joseph A., Behlke, Mark, Podyminogin, Mikhail
• Granted Patent: US 7,645,872 B2
• Time in Patent Office: Days
• US Class Current: 534/732
• CPC Class Codes: C07C 245/10 with nitrogen atoms of azo groups bound to carbon atoms of six-membered aromatic rings being part of condensed ring systems
• CPC Class Codes: C07H 21/04 with deoxyribosyl as saccharide radical
• CPC Class Codes: C12Q 1/6818 involving interaction of two or more labels, e.g. resonant energy transfer

Abstract

The invention provides a novel method of labeling oligonucleotides, with reporter moieties, including but not limited to, quenchers, fluorophores, biotin, digoxigenin, peptides and proteins. In addition, this invention provides a method of detecting hybridization of oligonucleotides. This invention also provides novel azo quenchers having the general formula shown below.

The invention further provides compositions comprising labeled oligonucleotides and solid supports. The invention also provides kits comprising at least one composition of the present invention.

Claims

1. A composition comprising a quencher linked to a compound selected from the group consisting of an antigen, a steroid, a vitamin, a drug, a hapten, a metabolite, a toxin, an environmental pollutant, an amino acid, a protein, a carbohydrate, a solid support, a linker, and a lipid, wherein the quencher is attached to the compound via an oxime bond.

2. The composition of claim 1, wherein the composition further comprises a fluorophore.

3. The composition of claim 1, wherein the quencher is a compound of Formula (I)

4. A kit comprising a composition of claim 1.

5. The kit of claim 4, further comprising instructions for the use of the composition.