Ultra Sensitive Tapered Fiber Optic Biosensor For Pathogens, Proteins, and DNA

• US 20090304551A1
• Filed: 01/26/2007
• Published: 12/10/2009
• Est. Priority Date: 01/31/2006
• Status: Abandoned Application

• Current Assignee: Drexel University
• Original Assignee: Drexel University
• Inventors: Shankar, Mohana P., Leung, Angela Shing Yan, Mutharasan, Rajakkannu
• Application Number:
  US12/162,447
• Publication Number:
  US 20090304551A1
• Time in Patent Office: Days
• US Class Current: 422/82.110
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 2021/7736 exposed, cladding free
• CPC Class Codes: G01N 2021/7786 Fluorescence
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/6408 with measurement of decay time, time resolved fluorescence
• CPC Class Codes: G01N 21/77 by observing the effect on a chemical indicator
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

A biconically tapered optical fiber serves as a biosensor at all optical wavelengths of interest ranging from UV to far IR at subfemtogram per mL sensitivity. The biconically tapered sensor detects biomaterials such as pathogenic species, proteins and DNA and others biological analytes. Although it uses the principles of evanescent fields, absorption, adsorption, fluorescence capture and retransmission through the fiber, the detection at the sub-nanogram per mL level is achieved primarily by adsorption or a surface activity due to a refractive index change. The geometry of the biconically tapered optical sensor affects its performance. The sensing modality is achieved in situ with a source connected at one end of a tapered fiber and a suitable detector at the other end. The tapered region is optionally immobilized with a recognition molecule such as an antibody to the target antigen or a complementary DNA strand. The sample is brought in contact with the tapered region either in batch mode or in a flow mode.

Claims

1. A tapered fiber capable of detecting an analyte in a liquid or vapor comprising:

an optical fiber having first and second ends and a tapered region having a diameter smaller than a diameter of said fiber at one of said ends, said tapered region being located between said first and second ends.

2. The tapered fiber of claim 1, wherein the tapered fiber is substantially cylindrical and comprises a waveguide inner core component and an outer cladding comprising an evanescent component.

3. The tapered fiber of claim 2, wherein the waveguide inner core comprises Ge-doped silica and the evanescent component comprises pure silica having a lower refractive index than the core.

4. The tapered fiber of claim 2, wherein the tapered fiber comprises a tapered grating that creates an optical resonator.

5. The tapered fiber of claim 1, wherein the tapered fiber is capable of detection at a sample concentration of less than 10 pg/mL.

6. The tapered fiber of claim 1, wherein the biosensor is capable of detection at a sample concentration of less than 10 fg/mL.

7. The tapered fiber of claim 1, wherein the tapered fiber is capable of detection at a wavelength of from about 850 nm to about 4000 nm.

8. The tapered fiber of claim 1, wherein the tapered fiber is capable of detection at a wavelength of from about 1100 nm to about 2500 nm.

9. The tapered fiber of claim 1, wherein the tapered fiber has a length greater than 0.1 mm and a waist diameter taken in the tapered portion of less than 25 m.

10. The tapered fiber of claim 1, wherein the tapered fiber has a waist diameter of from about 1 μ

m to about 25 μ

m taken in the tapered portion, and a taper length of about 0. 1 mm to about 10 mm.

11. The tapered fiber of claim 1, wherein the tapered fiber has a waist diameter of from about 1 μ

m to about 7 μ

m taken in the tapered portion, and a taper length of about 0.6 mm to about 1.2 mm.

12. The tapered fiber of claim 1, having a tapered portion that is engineered for detection of a specific analyte.

13. The tapered fiber of claim 12, wherein the tapered portion further comprises an antibody for a specific analyte bound to an inner surface of said tapered portion.

14. The tapered fiber of claim 1, wherein the tapered fiber is biconical.

15. A tapered fiber optic biosensor, comprising:

a tapered fiber as claimed in claim 1,

a light source operatively associated with said tapered fiber to provide light to the first end of said tapered fiber;

and at least one detector operatively associated with said tapered fiber for receiving light from the second end of said tapered fiber;

wherein the detector is capable of monitoring refractive index changes and the interaction between at least one analyte and an evanescent field in order to determine the presence and/or concentration of said analyte.

16. The tapered fiber optic biosensor of claim 15, wherein the biosensor is portable and microscaled.

17. The tapered fiber optic biosensor of claim 15, wherein the biosensor is enabled for in situ on line measurement of an optical throughput.

18. The tapered fiber optic biosensor of claim 15, wherein the biosensor is integrated in a device selected from the group consisting of glucose strips, pregnancy kits, MEMS, biopsy probes, endoscopes and catheters.

29. The tapered fiber of claim 1, wherein the tapered fiber has a waist diameter of from about 1 μ

m to about 15 μ

m taken in the tapered portion, and a taper length of about 0.5 mm to about 5 mm.

30. The tapered fiber of claim 1, wherein the tapered fiber has a waist diameter of from about 1 μ

m to about 25 μ

m taken in the tapered portion, and a taper length of about 0.1 mm to about 10 mm.

19-28. -28. (canceled)