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METHODS FOR AMPLIFYING NUCLEIC ACIDS AND FOR ANALYZING NUCLEIC ACIDS THEREWITH

  • US 20090305288A1
  • Filed: 06/18/2009
  • Published: 12/10/2009
  • Est. Priority Date: 12/21/2006
  • Status: Abandoned Application
First Claim
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1. A method for amplifying a nucleic acid, comprising:

  • a complementary strand amplifying step, in which a target double-stranded nucleic acid to be amplified, and a first primer complementary to a region in one strand of said nucleic acid are used;

    a second-primer adding step, in which a second primer complementary to a region on the 3′

    side of the amplified product from said complementary strand amplifying step is added;

    a double strand amplifying step in which said target double-stranded nucleic acid to be amplified is amplified in the presence of the first primer and the second primer.

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