Microdeposition System For A Biosensor

• US 20090305906A1
• Filed: 06/04/2008
• Published: 12/10/2009
• Est. Priority Date: 06/06/2007
• Status: Active Grant

• Current Assignee: Ascensia Diabetes Care Holdings AG (PHC Holdings Corporation)
• Original Assignee: Bayer Healthcare LLC (Bayer AG)
• Inventors: WU, Huan-Ping
• Granted Patent: US 9,182,393 B2
• Time in Patent Office: Days
• US Class Current: 506/13
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00387 Applications using probes
• CPC Class Codes: B01J 2219/00443 Thin film deposition
• CPC Class Codes: B01J 2219/00509 Microcolumns
• CPC Class Codes: B01J 2219/00603 Making arrays on substantially continuous surfaces
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00653 the compounds being bound to electrodes embedded in or on the solid supports
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 2400/025 tapping tip on substrate
• CPC Class Codes: B01L 3/0241 Drop counters; Drop formers making arrays for combinatorial libraries B01J19/0046; automation of dispensing for analysis G01N35/10
• CPC Class Codes: B01L 3/0248 Prongs, quill pen type dispenser
• CPC Class Codes: B01L 3/0275 Interchangeable or disposable dispensing tips
• CPC Class Codes: B01L 9/00 Supporting devices; Holding devices
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: G01N 2035/1037 Using surface tension, e.g. pins or wires
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

A microdeposition pin having a contact surface with at least one concave edge for creating microarrays and the like. The microdeposition pin may be used either alone or with a plurality of microdeposition pins in conjunction with a holder. The concave edge of the pin is especially adapted for helping to control the spreading of a deposited material. By selectively controlling the spread of the reagent composition from the microdeposition pin, the flow of the reagent composition from the deposition target area may be reduced. Sensor strips having raised substrate features with limited or no spreading of the reagent composition beyond the target area are disclosed.

Claims

1. A microdeposition pin, comprising:

a shaft terminating in a head, the head having a contact surface including at least one concave edge.

2. The pin of claim 1, the contact surface including at least two concave edges.

3. The pin of claim 1, wherein said contact surface includes a flat edge.

4. The pin of claim 1, wherein said contact surface includes a convex edge.

5. The pin of claim 1, wherein said head includes a first side wall that is approximately perpendicular to said contact surface.

6. The pin of claim 1, wherein said head includes a first side wall that is angled relative to said contact surface of the pin.

7. The pin of claim 1, wherein said head includes a concave side wall intersecting said contact surface to form said at least one concave edge, wherein said concave side wall extends substantially the length of said head.

8. The pin of claim 1, wherein said head includes a concave side wall intersecting said contact surface to form said at least one concave edge, wherein said concave side wall is tapered along a length of said head.

9. The pin of claim 1, wherein said head includes a channel therein terminating at an aperture disposed at said contact surface.

10. The pin of claim 1, further comprising a collar remote from said head of said pin, said collar adapted for affixing said pin to a holder.

11. The pin of claim 1, the cross-section of the contact surface including at least two convex and at least two concave exterior surfaces, the cross-section of the contact surface having a maximum diameter from about 0.25 mm to about 2.8 mm, and the cross-section of the contact surface having a minimum distance from about 0.1 mm to about 1.5 mm.

12. The pin of claim 1, the cross-section of the contact surface including at least two convex and at least two concave exterior surfaces, the cross-section of the contact surface having a ratio of the maximum diameter to the minimum distance of from 30 to 0.17.

13. The pin of claim 1, the cross-section of the contact surface having

a maximum diameter from about 2 mm to about 3 mm,

a minimum distance from about 1 mm to about 1.5 mm, and

a maximum arc distance from about 1.3 mm to about 1.4 mm,

where a radius defines the arc of the concave exterior surface and the radius has a length from about 3 mm to about 4 mm.

14. A microdeposition pin, comprising:

a shaft terminating in a contact surface having a polygonal cross-section with at least one convex exterior surface and at least one substantially flat exterior surface.

15. A biosensor, comprising:

a substrate having at least one raised feature; and

at least one reagent composition deposition on the raised feature, the deposition including an active biomolecule having a weight average molecular weight from about 10,000 to about 500,000,

where the spread of the reagent composition beyond the raised feature is at most 0.3 mm.

16. The biosensor of claim 15, the deposition including an active biomolecule having a weight average molecular weight from about 100,000 to about 400,000.

20. The biosensor of claim 15, where the raised feature has an average height above a top surface of the substrate of from about 5 μ

m to about 25 μ

m.

17-19. -19. (canceled)

21-38. -38. (canceled)

39. An apparatus for the deposition of a reagent composition, the apparatus comprising:

at least one microdeposition pin having a contact surface for engaging a substrate, wherein said contact surface of at least one of the microdeposition pins includes a concave edge; and

a holder for grasping said at least one microdeposition pin such that said contact surface is remote from said holder.

40. (canceled)

41. A method for creating a biosensor comprising:

dipping the contact surface of at least one microdeposition pin into reagent composition;

contacting a substrate with the at least one pin; and

depositing at least a portion of the reagent composition from the at least one pin to the substrate to form a substrate deposition, the substrate deposition having at least one substantially straight edge.

42. (canceled)

43. A method of forming a reagent composition footprint having at least one straight edge comprising:

depositing an amount of the reagent composition onto a contact surface of a pin, said contact surface having at least one concave edge; and

engaging said contact surface of said pin with a substrate.