Method and System for Multiplex Genetic Analysis

• US 20090305909A1
• Filed: 06/11/2009
• Published: 12/10/2009
• Est. Priority Date: 06/10/2005
• Status: Abandoned Application

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Woudenberg, Timothy, Oldham, Mark F., Nordman, Eric S.
• Application Number:
  US12/482,815
• Publication Number:
  US 20090305909A1
• Time in Patent Office: Days
• US Class Current: 506/32
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: C12Q 1/6874 involving nucleic acid arrays, e.g. sequencing by hybridisation
• CPC Class Codes: C12Q 2563/107 fluorescence
• CPC Class Codes: G01N 2021/6441 with two or more labels
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 21/648 using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence

Abstract

A method for selectively immobilizing a molecule of interest on a substrate is disclosed. A substrate having a first surface component and a second surface component is provided. The first and second surface components have different surface characteristics. The molecule of interest is selectively coupled to the first surface component based upon a difference between the surface characteristics of the first surface component and the surface characteristics of the second surface component.

Claims

1. A method of selectively immobilizing a molecule of interest on a substrate, comprising:

providing a substrate having a first surface component and a second surface component where the first and second surface component have different surface characteristics; and

selectively coupling the molecule of interest to the first surface component, based upon a difference between the surface characteristics of the first surface component and the surface characteristics of the second surface component.

2. The method of claim 1, wherein the first surface component comprises SiO₂ and the second surface component comprises a metal or metal oxide.

3. The method of claim 2, wherein the differing surface characteristics comprise surface charge.

4. The method of claim 1, wherein the substrate comprises a layer of the second surface component on a layer of the first surface component, and wherein the substrate comprises a zero mode waveguide disposed through the surface component layer to the first surface component layer.

5. The method of claim 1, wherein the molecule of interest associates preferentially to the first surface component.

6. The method of claim 1, wherein the substrate is contacted with a first composition that selectively associates with the first surface component based upon the difference between the surface characteristics of the first and second surface components.

7. The method of claim 6, wherein the first composition comprises a first coupling group, and wherein selectively coupling the molecule of interest to the first surface component comprises coupling the molecule of interest to the first coupling group.

8. The method of claim 7, wherein the first coupling group comprises biotin.

9. The method of claim 1, comprising contacting the substrate with a second composition that selectively associates with the second surface component based upon the difference between the surface characteristics of the first and second surface components.

10. The method of claim 9, wherein the second composition comprises a second coupling group.