Compositions and methods for control of insect infestations in plants
First Claim
1. A method for controlling invertebrate pest infestation comprising providing in the diet of an invertebrate pest an agent comprising a ribonucleic acid that functions upon ingestion by the pest to inhibit the expression of a target sequence within said pest, wherein said ribonucleic acid consists of a ribonucleotide sequence that is or is complementary to said target sequence, wherein said ribonucleotide sequence is transcribed from a DNA sequence selected from the group consisting of SEQ ID NO:
- 1 through SEQ ID NO;
143, SEQ ID NO;
169 through SEQ ID NO;
174, and the complement thereof.
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Abstract
The present invention is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The invention discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.
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Citations
63 Claims
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1. A method for controlling invertebrate pest infestation comprising providing in the diet of an invertebrate pest an agent comprising a ribonucleic acid that functions upon ingestion by the pest to inhibit the expression of a target sequence within said pest, wherein said ribonucleic acid consists of a ribonucleotide sequence that is or is complementary to said target sequence, wherein said ribonucleotide sequence is transcribed from a DNA sequence selected from the group consisting of SEQ ID NO:
- 1 through SEQ ID NO;
143, SEQ ID NO;
169 through SEQ ID NO;
174, and the complement thereof.
- 1 through SEQ ID NO;
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2-45. -45. (canceled)
- 46. A stacked transgenic event for reducing or eliminating plant pest infestation, comprising a dsRNA for suppression of an essential gene in a target pest and a pesticide exhibiting biological activity against said target pest.
- 59. A crop harvested from a field comprising the stacked transgenic event of 47, wherein said crop comprises said dsRNA and said pesticide.
Specification