Electrodes with Multilayer Membranes and Methods of Using and Making the Electrodes

• US 20090308743A1
• Filed: 08/20/2009
• Published: 12/17/2009
• Est. Priority Date: 05/12/2000
• Status: Abandoned Application

• Current Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories)
• Original Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories)
• Inventors: Heller, Adam, Friedman, Keith A., Chen, Ting
• Application Number:
  US12/544,689
• Publication Number:
  US 20090308743A1
• Time in Patent Office: Days
• US Class Current: 204/403.100
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001

Abstract

A sensor including a sensing layer is disposed over an electrode or an optode and a layer-by-layer assembled mass transport limiting membrane disposed over the sensing layer. The membrane includes at least one layer of a polyanionic or polycationic material. The assembled layers of the membrane are typically disposed in an alternating manner. The sensor also optionally includes a biocompatible membrane.

Claims

1-20. -20. (canceled)

21. A membrane structure, comprising:

a sensing layer composition comprising an analyte responsive enzyme and a redox polymer; and

a flux limiting membrane composition comprising a polymer comprising pyridines,

wherein the flux limiting membrane is disposed on and bound to the sensing layer.

22. The membrane structure of claim 21, wherein the membrane structure is disposed on a wire.

23. The membrane structure of claim 21, wherein the membrane structure is disposed on a substrate.

24. The membrane structure of claim 21, wherein the analyte responsive enzyme is a glucose responsive enzyme.

25. The membrane structure of claim 21, wherein the redox polymer comprises an osmium mediator complex.

26. The membrane structure of claim 21, wherein the redox polymer comprises a non-leachable redox compound.

27. The membrane structure of claim 21, further comprising a biocompatible layer disposed over the flux limiting membrane.

28. The membrane structure of claim 21, wherein the flux limiting membrane is configured and arranged to reduce flux of at least one interferent to the sensing layer.

29. The membrane structure of claim 21, wherein the flux limiting membrane is sufficient to limit a flux of glucose or lactate thereacross.

30. The membrane structure of claim 21, wherein the membrane structure has an active area of 0.1 cm or less.

31. The membrane structure of claim 30, wherein the active area is in a range of 10⁻

2 cm² to 10<sup>−

8</sup> cm².

32. The membrane structure of claim 30, wherein the active area is in a range of 10⁻

3 cm² to 10<sup>−

6</sup> cm².

33. A sensor, comprising:

an electrode;

a sensing layer composition comprising an analyte responsive enzyme and a redox polymer substrate disposed on the electrode; and

a flux limiting membrane composition comprising a polymer comprising pyridines,

wherein the flux limiting membrane is disposed on and bound to the sensing layer.

34. The sensor of claim 33, wherein the electrode is a wire.

35. The sensor of claim 33, wherein the electrode is disposed on a substrate.

36. The sensor of claim 33, wherein the analyte responsive enzyme is a glucose responsive enzyme.

37. The sensor of claim 33, wherein the redox polymer comprises an osmium mediator complex.

38. The sensor of claim 33, wherein the redox polymer comprises a non-leachable redox compound.

39. The sensor of claim 33, further comprising a biocompatible layer disposed over the flux limiting membrane.

40. The sensor of claim 33, wherein the flux limiting membrane is configured and arranged to reduce flux of at least one interferent to the sensing layer.

41. The sensor of claim 33, wherein the flux limiting membrane is sufficient to limit a flux of glucose or lactate thereacross.

42. The sensor of claim 33, wherein the sensor has an active area of 0.1 cm² or less.

43. The sensor of claim 42, wherein the active area is in a range of 10⁻

2 cm² to 10<sup>−

8</sup> cm².

44. The sensor of claim 42, wherein the active area is in a range of 10⁻

3 cm² to 10<sup>−

6</sup> cm².

45. The sensor of claim 33, wherein the sensor is adapted for implantation of at least a portion of the sensor in an animal.