CHROMATOGRAPHY DEVICE

• US 20090308811A1
• Filed: 06/11/2009
• Published: 12/17/2009
• Est. Priority Date: 06/11/2008
• Status: Abandoned Application

• Current Assignee: Argonide Corporation
• Original Assignee: Argonide Corporation
• Inventors: Kaledin, Leonid A., Kaledin, Tatiana, Tepper, Frederick
• Application Number:
  US12/482,585
• Publication Number:
  US 20090308811A1
• Time in Patent Office: Days
• US Class Current: 210/656
• CPC Class Codes: B01J 20/06 comprising oxides or hydroxides of metals not provided for in group B01J20/04
• CPC Class Codes: B01J 20/08 comprising aluminium oxide or hydroxide; comprising bauxite
• CPC Class Codes: B01J 20/103 comprising silica
• CPC Class Codes: B01J 20/28007 with size in the range 1-100 nanometers, e.g. nanosized particles, nanofibers, nanotubes, nanowires or the like carbon nanostructures B01J20/205
• CPC Class Codes: B01J 20/28028 Particles immobilised within fibres or filaments
• CPC Class Codes: B01J 20/282 Porous sorbents ion exchange B01J39/00 - B01J41/00
• CPC Class Codes: B01J 20/286 Phases chemically bonded to a substrate, e.g. to silica or to polymers
• CPC Class Codes: B01J 20/3204 Inorganic carriers, supports or substrates
• CPC Class Codes: B01J 20/3212 consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
• CPC Class Codes: B01J 20/3236 containing metal, other than zeolites, e.g. oxides, hydroxides, sulphides or salts
• CPC Class Codes: B01J 20/3274 Proteins, nucleic acids, polysaccharides, antibodies or antigens
• CPC Class Codes: B01J 20/3289 Coatings involving more than one layer of same or different nature
• CPC Class Codes: B01J 20/3293 Coatings on a core, the core being particle or fiber shaped, e.g. encapsulated particles, coated fibers
• CPC Class Codes: B01J 2220/54 Sorbents specially adapted for analytical or investigative chromatography
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: G01N 2030/524 structural properties

Abstract

A chromatography device and method of use to separate components of a sample are described. The device includes a stationary phase supported by a frame or contained within a chamber in a housing. The stationary phase includes a nano alumina medium that has support fibers having nano alumina fibers attached thereto. Optionally, sorbents are electrostatically adhered to the nano alumina fibers. Chromatographic separations are effected by the mobile phase at pressures of less than 10 bar and at flow velocities up to at least 5 cm/min. An electrical potential can be applied across the medium to foster separation of components.

Claims

1. A chromatography column, comprising:

a housing having an inlet and an outlet and a chamber positioned therebetween; and

a stationary phase contained within said chamber, said stationary phase including a nano alumina medium, said medium comprising support fibers having attached thereto nano alumina fibers, said support fibers having a diameter greater than that of said nano alumina fibers.

2. The chromatography column as in claim 1 wherein said stationary phase is wrapped around a support means.

3. The chromatography column as in claim 1 wherein said support fibers are selected from the group consisting of microglass and lyocell.

4. The chromatography column as in claim 1 wherein said nano alumina medium further comprises sorbent particles that electrostatically adhere to said nano alumina fibers.

5. The chromatography column as in claim 4 wherein said sorbent particles are selected from the group consisting of metal oxides, silica, and titania.

6. The chromatography column as in claim 4 wherein said sorbent particles are negative ions.

7. The chromatography column as in claim 4 wherein said sorbent particles are biological particles.

8. The chromatography column as in claim 1 wherein said nano alumina medium further comprises third fibers.

9. A chromatography column, comprising:

a housing having an inlet, an outlet, and a chamber positioned therebetween;

a perforated support means axially positioned within said chamber; and

a stationary phase contained within said chamber and wrapped around said support means, said stationary phase including a nano alumina medium, said medium comprising support fibers having attached thereto nano alumina fibers, said support fibers having a diameter greater than that of said nano alumina fibers.

10. The chromatography column as in claim 9 wherein said support fibers are selected from the group consisting of microglass and lyocell.

11. The chromatography column as in claim 9 wherein said nano alumina medium further comprises sorbent particles electrostatically adhered to said nano alumina fibers.

12. The chromatography column as in claim 9 wherein said nano alumina medium further comprises third fibers.

13. A chromatography device, comprising:

a frame, and

a stationary phase supported by said frame, said stationary phase including a nano alumina medium, said medium comprising support fibers having attached thereto nano alumina fibers, said support fibers having a diameter greater than that of said nano alumina fibers.

14. The chromatography device as in claim 13 further comprising a conduction means for conducting an electrical potential across said stationary phase.

15. The chromatography device as in claim 14 wherein said electrical potential is applied in a plane that is transverse to a plane in which said stationary phase is positioned.

16. The chromatography device as in claim 13 wherein said stationary phase includes a plurality of layers of said nano alumina medium.

17. The chromatography device as in claim 13 wherein said support fibers are selected from the group consisting of microglass and lyocell.

18. The chromatography device as in claim 13 wherein said nano alumina medium further comprises sorbent particles electrostatically adhered to said nano alumina fibers.

19. The chromatography device as in claim 13 wherein said nano alumina medium further comprises third fibers.

20. A method of chromatographic separation of a sample into separate components comprising the steps of:

a. placing a sample into a chromatography column, said chromatography column having a nano alumina medium, said medium comprising support fibers having attached thereto nano alumina fibers, said support fibers having a diameter greater than that of said nano alumina fibers;

b. passing a mobile phase through said chromatography column; and

c. eluting said separated components from said nano alumina medium

21. The method of chromatographic separation as set forth in claim 20, further comprising the step of applying an electrical potential across said stationary phase to foster separation of components.

22. The method of chromatographic separation as set forth in claim 20, wherein said step of placing said sample is carried out by placing said sample through said column in a first direction and said step of passing said mobile phase is carried out by placing said mobile phase through said column in a second direction that is opposite to said first direction.

23. The method of chromatographic separation as set forth in claim 20, wherein said components are selected from the group consisting of water soluble molecules, dyes, pollutants, proteins, peptides, nucleic acids, viruses, bacteria, water soluble molecules, soluble dyes, RNA, DNA, oligonucleotides, enzymes, antibodies, and antigens.

24. The method of chromatographic separation as set forth in claim 20, wherein said mobile phase is selected from the group consisting of an aqueous solution, an inorganic salt solution, and a polar solvent.

25. The method of chromatographic separation as set forth in claim 20, wherein said step of eluting is performed at a pressure drop of less than about 10 bar.