Test-Sensor Production Monitoring Using XRF Spectrometry

• US 20090310743A1
• Filed: 04/05/2007
• Published: 12/17/2009
• Est. Priority Date: 04/11/2006
• Status: Active Grant

• Current Assignee: Ascensia Diabetes Care Holdings AG (PHC Holdings Corporation)
• Original Assignee: Bayer Healthcare LLC (Bayer AG)
• Inventors: Wu, Huan-Ping, Carpenter, Scott E., Rey, Theresa A.
• Granted Patent: US 8,005,188 B2
• Time in Patent Office: Days
• US Class Current: 378/45
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: G01N 2223/076 X-ray fluorescence
• CPC Class Codes: G01N 23/223 by irradiating the sample with X-rays or gamma-rays and by measuring X-ray fluorescence
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52

Abstract

A sensor for determining the presence of an analyte in a test sample, said sensor comprising a nanoparticulate membrane comprising nanoparticles of at least one inorganic oxide of an element selected from Group IA, IIA, IIIA, IVA, IB, IIB, IIIB, IVAB, VB, VIB, VIII3 or V11113 of the Periodic Table, and wherein an oxidoreductase and an electrochemical activator are diffusibly dispersed in said nanoparticulate membrane.

Claims

1. A method for on-line monitoring of test sensors using XRF spectrometry, the method comprising the acts of:

providing a test sensor comprising a reagent, the reagent including an enzyme and a mediator, the mediator including a metallic element; and

monitoring the reagent of the test sensor using XRF spectrometry.

2. The method of claim 1, wherein the test sensor is an electrochemical test sensor.

3. The method of claim 1, wherein the test sensor is an optical test sensor.

4. The method of claim 1, wherein the mediator is a ferricyanide derivative and wherein the monitoring of the reagent of the test sensor includes measuring the iron.

5. The method of claim 1, wherein the ferricyanide derivative is potassium ferricyanide

6. The method of claim 1, wherein the mediator is a ferrocene derivative.

7. The method of claim 6, wherein the ferrocene derivative is 1,1′

dimethylferrocene, hydroxymethylferrocene, 2-hydroxyethylferrocene or carboxylateferrocene.

8. The method of claim 1, wherein the mediator is ruthenium hexaamine.

9. The method of claim 1, wherein the enzyme is a glucose-specific enzyme.

10. The method of claim 8, wherein the enzyme is a glucose oxidase.

11. A method for on-line monitoring of electrochemical test sensors using XRF spectrometry, the method comprising the acts of:

providing an electrochemical test sensor comprising a reagent, the reagent including an enzyme and a mediator, the mediator including a metallic element; and

monitoring the reagent of the test sensor using XRF spectrometry.

12. The method of claim 11, wherein the mediator is a ferricyanide derivative and wherein the monitoring of the reagent of the test sensor includes measuring the iron.

13. The method of claim 11, wherein the ferricyanide derivative is potassium ferricyanide.

14. The method of claim 11, wherein the mediator is a ferrocene derivative.

15. The method of claim 14, wherein the ferrocene derivative is 1,1′

dimethylferrocene, hydroxymethylferrocene, 2-hydroxyethylferrocene or carboxylateferrocene.

16. The method of claim 11, wherein the mediator is ruthenium hexaamine.

17. The method of claim 11, wherein the enzyme is a glucose-specific enzyme.

18. The method of claim 17, wherein the enzyme is a glucose oxidase.