Method for molecular delivery into cells using naonotube spearing
First Claim
1. A method of delivering a macromolecule to a B cell comprising:
- engaging a macromolecule to at least one nanotube wherein the nanotube comprises a magnetic particle;
placing the nanotubes into a sample wherein the sample comprises a plurality of cells;
applying a force to the sample wherein the force causes the nanotubes to collide with the cells to spear the cells; and
applying a second force which drives the nanotubes into the cell.
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Abstract
The present invention discloses a method of delivering a macromolecule to a cell, comprising engaging the macromolecule to a nanotube wherein the nanotube comprises a magnetic particle, placing the nanotubes into a sample wherein the sample comprises a plurality of cells and applying a force to the sample wherein the force causes the nanotubes to collide with the cells and thereby spear the cells. The method further discloses applying a second force which drives the nanotubes into the cell. Furthermore, the invention discloses a method of transfecting and/or transducing a cell, comprising engaging a macromolecule to a nanotube, placing the nanotubes into communication with the cells and applying a force to the nanotubes which causes the nanotubes to collide with the cells and thereby pierce the cellular membrane. Once inside the cell, the macromolecule may disengage from the nanotube.
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Citations
29 Claims
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1. A method of delivering a macromolecule to a B cell comprising:
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engaging a macromolecule to at least one nanotube wherein the nanotube comprises a magnetic particle; placing the nanotubes into a sample wherein the sample comprises a plurality of cells; applying a force to the sample wherein the force causes the nanotubes to collide with the cells to spear the cells; and applying a second force which drives the nanotubes into the cell. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method of transfecting a B cell comprising:
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engaging a macromolecule to at least one nanotube wherein the nanotube comprises a magnetic particle; placing the nanotubes into a sample wherein the sample comprises a plurality of cells; applying a force to the sample wherein the force causes the nanotubes to collide with the cells to spear the cells; applying a second force which drives the nanotubes into the cells; and disengaging the macromolecule from the nanotube. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19)
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20. A method of transducing a B cell comprising:
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engaging a macromolecule to a nanotube wherein the nanotube comprises a magnetic particle; placing the nanotubes into communication with a plurality of cells; applying a force to the nanotubes which causes the nanotubes to collide with the cells and pierce the cellular membrane; and disengaging the macromolecule from the nanotube.
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21. (canceled)
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22. (canceled)
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23. (canceled)
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24. (canceled)
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25. (canceled)
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26. (canceled)
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27. (canceled)
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29. (canceled)
Specification