BIOENGINEERED TISSUE CONSTRUCTS AND CARDIAC USES THEREOF
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Abstract
Cultured tissue constructs comprising cultured cells and endogenously produced extracellular matrix components without the requirement of exogenous matrix components or network support or scaffold members. Some tissue constructs of the invention are comprised of multiple cell layers or more than one cell type. The tissue constructs of the invention have morphological features and functions similar to tissues their cells are derived and their strength makes them easily handleable. Preferred cultured tissue constructs of the invention are prepared in defined media, that is, without the addition of chemically undefined components. These tissue constructs are used to repair cardiac tissues.
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Citations
51 Claims
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1-37. -37. (canceled)
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38. An occluder for a percutaneous transluminal procedure, comprising:
- an overall support structure; and
at least one occlusion shell, connected to the overall support structure, comprising a cultured tissue construct comprising fibroblast cells grown under conditions to produce a layer of extracellular matrix which is synthesized and assembled by the cultured fibroblast cells, with the cultured fibroblast cells contained within the synthesized extracellular matrix layer, and, a substance for stimulating tissue growth.
- an overall support structure; and
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39. A method for percutaneous transluminal closure of a cardiac opening in a patient, comprising:
- inserting an occluder into a heart of the patient, the occluder comprising;
an overall support structure; and
at least one occlusion shell connected to the overall support structure and comprising a cultured tissue construct comprising fibroblast cells grown under conditions to produce a layer of extracellular matrix which is synthesized and assembled by the cultured fibroblast cells, with the cultured fibroblast cells contained within the synthesized extracellular matrix layer, and;
positioning the occluder at least partially within the cardiac opening to substantially occlude the cardiac opening. - View Dependent Claims (40, 41, 42, 43)
- inserting an occluder into a heart of the patient, the occluder comprising;
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44. A method for promoting vascularization of a mammalian tissue in vivo, comprising:
- attaching a cell-matrix construct to the mammalian tissue to increase the number of blood vessels in the mammalian tissue, said cell-matrix construct comprising fibroblast cells and endogenously produced extracellular matrix components naturally secreted by the fibroblast cells.
- View Dependent Claims (45, 46, 47, 48)
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49. A method for promoting healing of a site of anastomosis in a subject, comprising:
- attaching a cell-matrix construct to the site to promote growth of endothelial cells and increase the number of blood vessels in the site, wherein said cell-matrix construct comprises fibroblast cells and endogenously produced extracellular matrix components naturally secreted by the fibroblast cells.
- View Dependent Claims (50, 51)
Specification