BIOENGINEERED TISSUE CONSTRUCTS AND CARDIAC USES THEREOF

• US 20090317441A1
• Filed: 02/07/2007
• Published: 12/24/2009
• Est. Priority Date: 02/07/2006
• Status: Abandoned Application

• Current Assignee: Organogenesis Inc. (Organogenesis Holdings, Inc.)
• Original Assignee: Organogenesis Inc. (Organogenesis Holdings, Inc.)
• Inventors: Eklund, Dario C., Bilbo, Patrick R.
• Application Number:
  US12/278,701
• Publication Number:
  US 20090317441A1
• Time in Patent Office: Days
• US Class Current: 424/423
• CPC Class Codes: A61B 17/00491 Surgical glue applicators surgical adhesives A61L24/00; two-component delivery syringes A61M5/19
• CPC Class Codes: A61B 17/0057 Implements for plugging an opening in the wall of a hollow or tubular organ, e.g. for sealing a vessel puncture or closing a cardiac septal defect sutures A61B17/04; closing rectum or urethra near body surface A61F2/0009
• CPC Class Codes: A61B 17/12122 within the heart for closing a cardiac septal defect A61B17/0057
• CPC Class Codes: A61B 17/12172 having a pre-set deployed three-dimensional shape methods of manufacturing A61B2017/00526
• CPC Class Codes: A61B 2017/00575 for closure at remote site, e.g. closing atrial septum defects
• CPC Class Codes: A61B 2017/00592 Elastic or resilient implements
• CPC Class Codes: A61B 2017/00597 Implements comprising a membrane
• CPC Class Codes: A61B 2017/00606 Implements H-shaped in cross-section, i.e. with occluders on both sides of the opening
• CPC Class Codes: A61B 2017/00831 Material properties
• CPC Class Codes: A61B 2017/00893 pharmaceutically effective
• CPC Class Codes: A61B 2017/1205 Introduction devices
• CPC Class Codes: A61L 2430/36 for embolization or occlusion, e.g. vaso-occlusive compositions or devices artificial blood vessels A61L27/507
• CPC Class Codes: A61L 27/3633 Extracellular matrix [ECM]
• CPC Class Codes: A61L 27/3804 characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
• CPC Class Codes: A61L 27/3839 characterised by the site of application in the body materials for artificial blood vessels A61L27/507; materials for use in artificial skin A61L27/60
• CPC Class Codes: A61L 27/3895 using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
• CPC Class Codes: A61L 31/125 having a macromolecular matrix
• CPC Class Codes: A61L 31/16 Biologically active materials, e.g. therapeutic substances A61L31/047 takes precedence

Abstract

Cultured tissue constructs comprising cultured cells and endogenously produced extracellular matrix components without the requirement of exogenous matrix components or network support or scaffold members. Some tissue constructs of the invention are comprised of multiple cell layers or more than one cell type. The tissue constructs of the invention have morphological features and functions similar to tissues their cells are derived and their strength makes them easily handleable. Preferred cultured tissue constructs of the invention are prepared in defined media, that is, without the addition of chemically undefined components. These tissue constructs are used to repair cardiac tissues.

Claims

1-37. -37. (canceled)

38. An occluder for a percutaneous transluminal procedure, comprising:

an overall support structure; and

at least one occlusion shell, connected to the overall support structure, comprising a cultured tissue construct comprising fibroblast cells grown under conditions to produce a layer of extracellular matrix which is synthesized and assembled by the cultured fibroblast cells, with the cultured fibroblast cells contained within the synthesized extracellular matrix layer, and, a substance for stimulating tissue growth.

39. A method for percutaneous transluminal closure of a cardiac opening in a patient, comprising:

inserting an occluder into a heart of the patient, the occluder comprising;

an overall support structure; and

at least one occlusion shell connected to the overall support structure and comprising a cultured tissue construct comprising fibroblast cells grown under conditions to produce a layer of extracellular matrix which is synthesized and assembled by the cultured fibroblast cells, with the cultured fibroblast cells contained within the synthesized extracellular matrix layer, and;

positioning the occluder at least partially within the cardiac opening to substantially occlude the cardiac opening.

40. The method of claim 39, wherein the overall support structure of the occluder comprises a proximal support structure and a distal support structure, the proximal support structure connecting to a proximal occlusion shell and the distal support structure connecting to a distal occlusion shell, and wherein positioning the occluder at least partially within the cardiac opening comprises positioning a portion of the overall support structure within the cardiac opening and positioning the proximal occlusion shell and the distal occlusion shell on different sides of the cardiac opening.

41. The method of claim 39, wherein the cardiac opening is a patent foramen ovale.

42. The method of claim 39, wherein the cardiac opening is an atrial septal defect.

43. The method of claim 39, wherein the cardiac opening is a ventricular septal defect.

44. A method for promoting vascularization of a mammalian tissue in vivo, comprising:

attaching a cell-matrix construct to the mammalian tissue to increase the number of blood vessels in the mammalian tissue, said cell-matrix construct comprising fibroblast cells and endogenously produced extracellular matrix components naturally secreted by the fibroblast cells.

45. The method of claim 44, wherein the mammalian tissue is cardiac tissue, skeletal muscle, smooth muscle, connective tissue or skin tissue.

46. The method of claim 44, wherein the cell-matrix construct is adhered to the mammalian tissue by natural cellular attachment.

47. The method of claim 46, wherein the cell-matrix construct is attached to the mammalian tissue by an attachment means.

48. The method of claim 47, wherein the attachment means is a suture, a biologic glue, a synthetic glue, a laser dye or a hydrogel.

49. A method for promoting healing of a site of anastomosis in a subject, comprising:

attaching a cell-matrix construct to the site to promote growth of endothelial cells and increase the number of blood vessels in the site, wherein said cell-matrix construct comprises fibroblast cells and endogenously produced extracellular matrix components naturally secreted by the fibroblast cells.

50. The method of claim 49, wherein the cell-matrix construct is adhered to the site by natural cellular attachment.

51. The method of claim 49, wherein the cell-matrix construct is attached to the site by any of a suture, a biologic glue, a synthetic glue, a laser dye, or a hydrogel.