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ANALYSIS USING MICROFLUIDIC PARTITIONING DEVICES

  • US 20090317798A1
  • Filed: 06/02/2006
  • Published: 12/24/2009
  • Est. Priority Date: 06/02/2005
  • Status: Abandoned Application
First Claim
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1. An assay method comprising:

  • (a) partitioning a sample obtained from the blood of a pregnant woman into a plurality of sub-samples,wherein said sample comprises a plurality of nucleic acid molecules, and wherein most of said sub-samples comprise either (i) 0 or 1 target nucleic acid sequences or (ii) 0 or 1 cells;

    (b) providing sufficient reagents in each sub-sample to amplify at least two different target sequences;

    (c) amplifying target sequence(s) in the two sub-sample(s)thereby producing amplicon(s) in the sub-sample(s);

    (d) querying the sub-samples for the presence of an amplicon, or a property of an amplicon, to obtain an indication of fetal disease or propensity to disease.

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