THERMUS EGERTSSONII DNA POLYMERASES
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Abstract
The present invention relates to a thermophilic polymerase, wherein the DNA polymerase has an in-vitro primer extension rate that is >35 bases/second and faster relative to the primer extension rate of a DNA polymerase comprising amino acid sequences SEQ ID NO: 2 or 4, when measured under identical conditions in a DNA replication assay using primed single strand M13mp18 DNA and an incubation temperature of 60° C. The invention also relates to a vector comprising the polymerase, a host cell comprising the vector. The invention relates to a nucleic acid replication kit comprising the polymerase according to the invention.
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Citations
25 Claims
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1-13. -13. (canceled)
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14. A DNA polymerase, wherein the DNA polymerase comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 6 and SEQ ID NO;
44. - View Dependent Claims (21, 22, 23, 24, 25)
- 6 and SEQ ID NO;
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15. A nucleic acid sequence encoding the amino acid sequence selected from the group consisting of SEQ ID NO:
- 6 and SEQ ID NO;
44. - View Dependent Claims (16, 19)
- 6 and SEQ ID NO;
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17. A nucleic acid sequence comprising a sequence selected from the group consisting of SEQ ID NO:
- 5 and SEQ ID NO;
43, or a functional fragment thereof. - View Dependent Claims (18, 20)
- 5 and SEQ ID NO;
Specification