Method of Measuring Glycated Hemoglobin Concentration

• US 20090317912A1
• Filed: 03/23/2007
• Published: 12/24/2009
• Est. Priority Date: 03/24/2006
• Status: Active Grant

• Current Assignee: ARKRAY, Inc
• Original Assignee: ARKRAY, Inc
• Inventors: Sugiyama, Koji, Sakai, Toshikatsu
• Granted Patent: US 8,021,887 B2
• Time in Patent Office: Days
• US Class Current: 436/67
• CPC Class Codes: G01N 2021/3137 with selection of wavelengths after the sample
• CPC Class Codes: G01N 2021/3144 for oxymetry
• CPC Class Codes: G01N 2030/8822 involving blood
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/314 with comparison of measurements at specific and non-specific wavelengths dual wavelength spectrometry G01J3/427
• CPC Class Codes: G01N 30/74 Optical detectors measurement of intensity, velocity, spectral content, polarisation, or phase of infrared, visible or ultraviolet light G01J
• CPC Class Codes: G01N 30/88 Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
• CPC Class Codes: G01N 33/723 Glycosylated haemoglobin
• CPC Class Codes: Y10T 436/25 including sample preparation

Abstract

The present invention relates to a method and an apparatus that measure the concentration of glycated hemoglobin by an optical technique. A wavelength in which the molecular extinction coefficient of oxyhemoglobin agrees or substantially agrees with the molecular extinction coefficient of deoxyhemoglobin is adopted as a measurement wavelength. Preferably, the measurement wavelength is set at from 417 to 421 nm. In the present invention, the concentration of glycated hemoglobin is measured by making use of column chromatography and of using a sample prepared from red blood cells in blood.

Claims

1. A method of measuring the concentration of glycated hemoglobin by an optical technique, wherein

the measurement wavelength is set at a wavelength in which the molecular extinction coefficient of oxyhemoglobin agrees or substantially agrees with that of deoxyhemoglobin.

2. The method of measuring the concentration of glycated hemoglobin according to claim 1, wherein

the measurement wavelength is set at from 417 to 427 nm.

3. The method of measuring the concentration of glycated hemoglobin according to claim 1, wherein

the measurement wavelength is set at from 419 to 425 nm.

4. The method of measuring the concentration of glycated hemoglobin according to claim 1, wherein

the measurement wavelength is set at from 520 to 526 nm or from 583 to 589 nm.

5. The method of measuring the concentration of glycated hemoglobin according to claim 1, wherein

the concentration of glycated hemoglobin is measured by column chromatography.

6. The method of measuring the concentration of glycated hemoglobin according to claim 1, wherein

the concentration of glycated hemoglobin is measured by use of a sample prepared from a red blood cell in blood.

7. An apparatus of measuring the concentration of glycated hemoglobin comprising a photometry mechanism in which a sample is irradiated with light from a light source and at the time a light receiving section receives light that travels from the sample, wherein

the photometry mechanism is configured to be capable of receiving light of a wavelength in which the molecular extinction coefficient of oxyhemoglobin agrees or substantially agrees with that of deoxyhemoglobin in the light receiving section.

8. The apparatus of measuring the concentration of glycated hemoglobin according to claim 7,

the photometry mechanism is configured to be capable of receiving light of from 417 to 427 nm in the light receiving section.

9. The apparatus of measuring the concentration of glycated hemoglobin according to claim 7,

the photometry mechanism is configured to be capable of receiving light of from 419 to 425 nm in the light receiving section.

10. The apparatus of measuring the concentration of glycated hemoglobin according to claim 7,

the photometry mechanism is configured to be capable of receiving light of from 520 to 526 nm or from 583 to 589 nm in a light receiving section.

11. The apparatus of measuring the concentration of glycated hemoglobin according to claim 7, further comprising:

a separation unit for separating glycated hemoglobin by column chromatography.

12. The apparatus of measuring the concentration of glycated hemoglobin according to claim 7, further comprising:

a sample preparation mechanism for preparing a sample from red blood cell in blood.