MICROFLUIDIC SELECTION OF LIBRARY ELEMENTS

• US 20090318303A1
• Filed: 06/20/2008
• Published: 12/24/2009
• Est. Priority Date: 06/20/2008
• Status: Abandoned Application

• Current Assignee: International Business Machines Corporation
• Original Assignee: International Business Machines Corporation
• Inventors: Lovchik, Robert, Delamarche, Emmanuel, Solis, Daniel J.
• Application Number:
  US12/143,314
• Publication Number:
  US 20090318303A1
• Time in Patent Office: Days
• US Class Current: 506/9
• CPC Class Codes: B01J 2219/00353 Pumps
• CPC Class Codes: B01J 2219/00414 using suction
• CPC Class Codes: B01J 2219/00418 using pressure
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00704 integrated with the reactor apparatus
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01J 2219/0074 Biological products
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/0631 Purification arrangements, e.g. solid phase extraction [SPE]
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0851 Bottom walls
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2300/12 Specific details about materials
• CPC Class Codes: B01L 2300/163 : Biocompatibility
• CPC Class Codes: B01L 2400/0406 : capillary forces
• CPC Class Codes: B01L 2400/049 : vacuum
• CPC Class Codes: B01L 3/502707 : characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502715 : characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: C40B 60/12 : for screening libraries
• CPC Class Codes: G01N 33/54366 : Apparatus specially adapted for solid-phase testing

Abstract

Disclosed herein is a system comprising a chip; a flow channel disposed in the chip; the flow channel being in communication with an entry port and an exit port; the flow channel being operative to permit the flow of a library from the entry port to the exit port; a substrate; the substrate being disposed upon the chip; the substrate being operative to act as an upper wall for the flow channel; and a receptor; the receptor being disposed on the substrate; the receptor being operative to interact with a component from the library.

Claims

1. A system comprising:

a chip;

a flow channel disposed in the chip;

the flow channel being in communication with an entry port and an exit port;

the flow channel being operative to permit the flow of a library from the entry port to the exit port;

a substrate;

the substrate being disposed upon the chip;

the substrate being operative to act as an upper wall for the flow channel; and

a receptor;

the receptor being disposed on the substrate;

the receptor being operative to interact with an element from the library.

2. The system of claim 1, wherein the flow channel is coated with a metal layer.

3. The system of claim 2, wherein the metal layer comprises gold.

4. The system of claim 1, wherein the chip comprises silicon.

5. The system of claim 1, wherein the chip comprises an organic polymer.

6. The system of claim 1, wherein the chip comprises a metal oxide;

the metal oxide being silica, alumina, titania, zirconia, ceria, or combinations comprising at least one of the foregoing metal oxides.

7. The system of claim 1, wherein the flow channel has a width of 30 to about 130 micrometers.

8. The system of claim 1, wherein the flow channel has a depth of about 10 to about 50 micrometers.

9. The system of claim 1, wherein the flow channel has a length of about 1 to about 150 millimeters.

10. The system of claim 1, wherein the flow channel has a path that is tortuous.

11. The system of claim 1, wherein the substrate comprises an elastomer;

the elastomer being a polysiloxane;

natural polyisoprene;

synthetic polyisoprene;

polybutadiene;

styrene butadiene copolymers;

copolymers of isobutylene and isoprene;

chlorobutyl rubber;

bromobutyl rubber;

copolymers of polybutadiene and acrylonitrile;

epichlorohydrin rubber;

polyacrylic rubber;

fluorosilicone rubber;

chlorosulfonated polyethylenes;

or a combination comprising at least one of the foregoing elastomers.

12. The system of claim 1, wherein the substrate comprises polydimethylsiloxane.

13. The system of claim 1, wherein the receptor comprises an enzyme, a peptide, a protein, an inorganic particle, a cell, a glycan, a viral particle, a polymer, an antibody, an antigen, or a combination comprising at least one of the foregoing receptors.

14. The system of claim 1, further comprising a pump;

the pump being in communication with the exit port.

15. The system of claim 1, further comprising a loading pad;

the loading pad being in communication with the entry port.

16. An article that uses the system of claim 1.

17. A method comprising:

disposing a library on a loading pad of a microfluidic device;

the microfluidic device comprising;

a chip;

a flow channel disposed in the chip;

the flow channel being in communication with an entry port and an exit port;

the flow channel being operative to permit the flow of a library from the entry port to the exit port;

a substrate;

the substrate being disposed upon the chip;

the substrate being operative to act as an upper wall for the flow channel; and

a receptor;

the receptor being disposed on the substrate;

the receptor being operative to interact with an element from the library;

adding a first solution to the loading pad to transport elements of the library through the entry port into the flow channel;

binding a fraction of the elements of the library to the receptor to form a element-receptor complex; and

eluting a element-receptor complex.

18. The method of claim 17, further comprising amplifying those elements of the library that are able to bind to the receptor.

19. The method of claim 17, further comprising analyzing those elements of the library that are able to bind to the receptor;

the analysis being conducted by analytical techniques;

the analytical techniques comprising oligonucleotide sequencing, radioactivity, fluorescence, chemiluminescence, phosphorescence, enzymatic activity, mass-spectroscopy, calorimetry, or a combination comprising at least one of the foregoing analytical techniques.

20. The method of claim 17, wherein the eluting of the element-receptor complex is accomplished using a second solution.

21. A method of manufacturing a microfluid device comprising:

disposing a flow channel in a chip;

disposing an exit port and a loading pad in the chip;

disposing a metal layer on a base of the flow channel;

disposing a substrate on the chip;

the substrate being operative to act as an upper wall for the flow channel; and

disposing a receptor on a surface of the substrate that is opposedly disposed to the metal layer;

the receptor being operative to interact with an element of a library.

22. The method of claim 21, wherein the chip is microfabricated.

23. The method of claim 21, wherein the chip does not seal an entry port;

the entry port being in communication with the loading pad.

24. An article manufactured by the method of claim 21.