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Efficient Shotgun Sequencing Methods

  • US 20090318304A1
  • Filed: 12/01/2008
  • Published: 12/24/2009
  • Est. Priority Date: 11/29/2007
  • Status: Active Grant
First Claim
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1. A method of sequencing a target nucleic acid comprising:

  • a. sequencing the target nucleic acid to produce a primary target nucleic acid sequence that comprises one or more sequences of interest;

    b. synthesizing a plurality of target-specific oligonucleotides, wherein each of said plurality of oligonucleotides corresponds to at least one of said sequences of interest;

    c. providing a library of fragments of the target nucleic acid that hybridize to said plurality of oligonucleotides;

    d. sequencing the library of fragments to produce a secondary target nucleic acid sequence.

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