INTEGRATED MICROFLUIDIC ASSAY DEVICES AND METHODS
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Abstract
Combinations of microfluidic diagnostic testing modules for simultaneous evaluations of serological and molecular biological targets are provided, and include panel testing for both antibodies (or antigens) and nucleic acid targets in one single-use device. These improvements are directed to evaluating the overall progress and activity of a pathogenic process in real time, at the point of care, not merely the presence or absence of a particular diagnostic marker, which can often be incomplete or misleading.
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Citations
84 Claims
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1-64. -64. (canceled)
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65. An apparatus for performing differential laboratory diagnostic testing, comprising:
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a) a disposable, single-entry microfluidic card having a plastic body and a sample inlet port for receiving a biological sample, said sample inlet port having a first valved fluidic connection to an immunoassay fluidic subcircuit and a second valved fluidic connection to a nucleic acid assay fluidic subcircuit; and b) a host instrument having a dock configured for receiving said microfluidic card and a microprocessor with user interface configured for pneumatically controlling a plurality of immunoassays, a plurality of nucleic acid assays, or a combination of assays thereof on said microfluidic card, under control of at least one command entered by a user, wherein said immunoassay fluidic subcircuit is configured with on-board reagents and means for performing a plurality of immunoassays and said nucleic acid assay fluidic subcircuit is configured with i) on-board reagents, ii) means for performing a plurality of nucleic acid assays, and iii) a nucleic acid extraction subcircuit. - View Dependent Claims (66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84)
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Specification