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Electrochemical Assay for the Detection of Enzymes

  • US 20100003710A1
  • Filed: 10/17/2008
  • Published: 01/07/2010
  • Est. Priority Date: 10/17/2007
  • Status: Abandoned Application
First Claim
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1. A method for detecting a protease in a test sample, said method comprising:

  • (a) adding a test sample comprising a protease to a solid support comprising an electrode comprising;

    (i) a self-assembled monolayer (SAM);

    (ii) a covalently attached eletroactive active moiety (EAM) comprising a transition metal complex with an E0, wherein said EAM comprises a ferrocene; and

    (iii) a plurality of proteins attached to said electrode, wherein said proteins comprise a cleavage site of said protease;

    (b) cleaving a plurality of said proteins with said protease; and

    (c) determining the presence of said protease by measuring a change of said E0.

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