Methods and compositions for generation of Bax-and Bak-deficient cell lines
First Claim
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1. A zinc finger DNA-binding domain comprising four, five or six zinc finger recognition regions as shown in Table 1 or Table 2.
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Abstract
Disclosed herein are methods and compositions for generation of Bak- and/or Bax-deficient cell lines using engineered nucleases.
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18 Claims
- 1. A zinc finger DNA-binding domain comprising four, five or six zinc finger recognition regions as shown in Table 1 or Table 2.
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7. A cell line in which Bak is partially or fully inactivated.
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8. A cell line in which Bax is partially or fully inactivated.
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9. A cell line in which Bak and Bax are partially or fully inactivated.
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10. A method of inactivating an endogenous cellular BAK or BAX gene in a cell, the method comprising:
(a) introducing, into a cell, a first nucleic acid encoding a first polypeptide, wherein the first polypeptide comprises; (i) a zinc finger DNA-binding domain that is engineered to bind to a first target site in an endogenous BAK or BAX gene; and (ii) a cleavage domain;
such that the polypeptide is expressed in the cell, whereby the polypeptide binds to the target site and cleaves at least one of the BAK and BAX genes.- View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18)
Specification