NOVEL GENES RELATED TO GLUTAMINYL CYCLASE
First Claim
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1. A method of screening for a compound capable of inhibiting an enzymatic activity, comprising:
- incubating at least a first polypeptide and a suitable substrate for the at least a first polypeptide in the presence of at least one test compound or salt thereof;
measuring an enzymatic activity of the at least a first polypeptide;
comparing said activity with enzymatic activity determined in the absence of the at least one test compound or salt thereof; and
selecting a test compound that reduces the enzymatic activity of the at least a first polypeptide;
wherein the at least a first polypeptide comprises;
(a) an amino acid sequence selected from the group consisting of SEQ ID NO;
11, SEQ ID NO;
12, SEQ ID NO;
13, SEQ ID NO;
14, SEQ ID NO;
15, SEQ ID NO;
16, SEQ ID NO;
17, SEQ ID NO;
18, SEQ ID NO;
21, and SEQ ID NO;
22, or an amino acid sequence having at least about 90% sequence identity thereto and having glutaminyl cyclase activity;
(b) an amino acid sequence encoded by a nucleic acid sequence selected from the group consisting of SEQ ID NO;
2, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
5, SEQ ID NO;
6, SEQ ID NO;
7, SEQ ID NO;
8, SEQ ID NO;
9, SEQ ID NO;
19, and SEQ ID NO;
20, or an alternative splice variant thereof;
or(c) a fragment of (a) or (b) wherein said fragment is immunologically reactive and has glutaminyl cyclase activity; and
the at least a first polypeptide is optionally glycosylated.
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Abstract
Novel glutaminyl-peptide cyclotransferase-like proteins (QPCTLs), which are isoenzymes of glutaminyl cyclase (QC, EC 2.3.2.5), and to isolated nucleic acids coding for these isoenzymes, all of which are useful for the discovery of new therapeutic agents, for measuring cyclase activity, and for determining the inhibitory activity of compounds against these glutaminyl cyclase isoenzymes.
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Citations
4 Claims
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1. A method of screening for a compound capable of inhibiting an enzymatic activity, comprising:
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incubating at least a first polypeptide and a suitable substrate for the at least a first polypeptide in the presence of at least one test compound or salt thereof; measuring an enzymatic activity of the at least a first polypeptide; comparing said activity with enzymatic activity determined in the absence of the at least one test compound or salt thereof; and selecting a test compound that reduces the enzymatic activity of the at least a first polypeptide; wherein the at least a first polypeptide comprises; (a) an amino acid sequence selected from the group consisting of SEQ ID NO;
11, SEQ ID NO;
12, SEQ ID NO;
13, SEQ ID NO;
14, SEQ ID NO;
15, SEQ ID NO;
16, SEQ ID NO;
17, SEQ ID NO;
18, SEQ ID NO;
21, and SEQ ID NO;
22, or an amino acid sequence having at least about 90% sequence identity thereto and having glutaminyl cyclase activity;(b) an amino acid sequence encoded by a nucleic acid sequence selected from the group consisting of SEQ ID NO;
2, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
5, SEQ ID NO;
6, SEQ ID NO;
7, SEQ ID NO;
8, SEQ ID NO;
9, SEQ ID NO;
19, and SEQ ID NO;
20, or an alternative splice variant thereof;
or(c) a fragment of (a) or (b) wherein said fragment is immunologically reactive and has glutaminyl cyclase activity; and the at least a first polypeptide is optionally glycosylated. - View Dependent Claims (2, 3, 4)
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Specification