BIOMOLECULE SENSOR, METHOD FOR MANUFACTURING THE SAME, BIOMOLECULE DETECTION METHOD, AND BIOMOLECULE DETECTION SYSTEM
First Claim
Patent Images
1. A biomolecule sensor, comprising:
- a carrier substrate; and
single probe molecules aligned and fixed respectively on grid points on the carrier substrate.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention aims to improve detecting accuracy and reproducibility of a biomolecule sensor. The biomolecule sensor of the present invention includes single probe molecules orderly aligned and fixed on grid points on the surface of a substrate. Accordingly, in the biomolecule sensor of the present invention: probe molecules for detecting a biomolecule are orderly aligned and separately fixed; blocking for preventing non-specific adsorption is applied to a region other than the region of the probe molecules for detecting a biomolecule; and fluorescence enhancement is achieved by metal microparticles.
-
Citations
14 Claims
-
1. A biomolecule sensor, comprising:
-
a carrier substrate; and single probe molecules aligned and fixed respectively on grid points on the carrier substrate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
-
-
9. A method for manufacturing a biomolecule sensor in which single probe molecules are aligned and fixed respectively on grid points on a carrier substrate, the method comprising the steps of:
-
fixing metal microparticles respectively on the grid points on the surface of the carrier substrate; fixing adsorption inhibitors on the surface of the substrate; and fixing the single probe molecules respectively on the surfaces of the metal microparticles. - View Dependent Claims (10, 11)
-
-
12. A biomolecule detection method using a biomolecule sensor including:
- metal microparticles fixed respectively on grid points on a carrier substrate; and
single probe molecules fixed respectively on the surfaces of the metal microparticles, the method comprising the steps of;causing the probe molecule of the biomolecule sensor and a fluorescently labeled sample biomolecule to react with each other; irradiating the biomolecule sensor after the reaction with an excitation light; and detecting fluorescence emitted from the region having the probe molecule fixed thereon.
- metal microparticles fixed respectively on grid points on a carrier substrate; and
-
13. A biomolecule detection method for detecting a nucleic acid sequence using a biomolecule sensor including:
- metal microparticles fixed respectively on grid points on a carrier substrate; and
single probe nucleic acid molecules fixed respectively on the surfaces of the metal microparticles, the method comprising the steps of;causing the probe nucleic acid molecule of the biomolecule sensor and a nucleic acid molecule to be sequenced to react with each other; causing the nucleic acid molecule to be sequenced and a fluorescently labeled nucleotide to react with each other; irradiating the biomolecule sensor with an excitation light; and detecting fluorescence emitted from the fluorescently labeled nucleotide.
- metal microparticles fixed respectively on grid points on a carrier substrate; and
-
14. A biomolecule detection system for detecting a nucleic acid sequence using a biomolecule sensor including:
- metal microparticles fixed respectively on grid points on a carrier substrate; and
single probe nucleic acid molecules fixed respectively on the surfaces of the metal microparticles, the biomolecule detection system comprising;a substrate serving as a platform for a reaction between the probe nucleic acid molecule of the biomolecule sensor and a nucleic acid molecule to be sequenced, and for a reaction between the nucleic acid molecule to be sequenced and a fluorescently labeled nucleotide; an irradiation source for irradiating the biomolecule sensor with an excitation light; and a detector for detecting fluorescence emitted from the fluorescently labeled nucleotide.
- metal microparticles fixed respectively on grid points on a carrier substrate; and
Specification