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Single molecule loading methods and compositions

  • US 20100009872A1
  • Filed: 03/30/2009
  • Published: 01/14/2010
  • Est. Priority Date: 03/31/2008
  • Status: Active Grant
First Claim
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1. A method of distributing a population of target molecules into a plurality of size confined reaction or observation regions, the method comprising:

  • providing a structure comprising the plurality of size confined reaction or observation regions;

    providing the population of target molecules to be distributed;

    adjusting the size of the confined reaction or observation regions by adding at least one sizing moiety to individual reaction or observation regions such that a selected number of target molecules will fit into the resulting size-adjusted regions, oradjusting the size of individual target molecules of the population by linking at least one sizing moiety to individual target molecules, wherein the sizing moieties are of sufficient size, relative to the size-confined reaction or observation regions, so that only a selected number of sizing moieties will fit into the size confined regions; and

    ,loading the target molecules into the regions, whereby a selected number of target molecules can fit into each region, thereby distributing the population of target molecules into the plurality of size confined regions.

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