CELL CARRIER AND CELL CARRIER CONTAINMENT DEVICES CONTAINING REGENERATIVE CELLS
First Claim
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1. A method of restoring cartilage, tendon or ligament in a patient, comprising:
- a) providing a tissue removal system and a prosthetic device comprising a cell carrier portion and a cell carrier containment portion, said cell carrier containment portion being configured to at least partially contain the cell carrier portion;
b) removing adipose tissue from the patient using the tissue removal system;
c) cryopreserving the adipose tissue;
c) processing the cryopreserved adipose tissue to obtain a concentrated population of cells comprising adipose-derived stem cells;
d) introducing the concentrated population of cells comprising adipose-derived stem cells to the cell carrier portion of the prosthetic device; and
e) inserting the prosthetic device containing the concentrated population of cells comprising adipose-derived stem cells into the intended bone formation area in the patient.
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Abstract
The present invention relates to a device comprising a cell carrier portion containing regenerative cells, e.g. stem and progenitor cells, and a cell carrier containment portion. The device is useful for the treatment of bone related disorders, including spinal fusion related disorders and long bone or flat bone related defects. The device may be used in conjunction with disclosed automated systems and methods for separating and concentrating regenerative cells.
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Citations
7 Claims
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1. A method of restoring cartilage, tendon or ligament in a patient, comprising:
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a) providing a tissue removal system and a prosthetic device comprising a cell carrier portion and a cell carrier containment portion, said cell carrier containment portion being configured to at least partially contain the cell carrier portion; b) removing adipose tissue from the patient using the tissue removal system; c) cryopreserving the adipose tissue; c) processing the cryopreserved adipose tissue to obtain a concentrated population of cells comprising adipose-derived stem cells; d) introducing the concentrated population of cells comprising adipose-derived stem cells to the cell carrier portion of the prosthetic device; and e) inserting the prosthetic device containing the concentrated population of cells comprising adipose-derived stem cells into the intended bone formation area in the patient.
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2. The method of claim 1, wherein cartilage, tendon or ligament is restored in the patient.
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3. A method of restoring cartilage, tendon or ligament in a patient comprising:
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a) providing a self contained adipose derived stem cell processing unit and a device comprising a cell carrier portion and a cell carrier containment portion, wherein said cell carrier containment portion is configured to at least partially contain the cell carrier portion, wherein said self contained adipose derived stem cell processing unit comprises a tissue collection chamber that is configured to receive unprocessed adipose tissue that is removed from said patient, wherein the tissue collection chamber is defined by a closed system; a first filter that is disposed within said tissue collection chamber, wherein said first filter is configured to substantially retain a first component of said unprocessed adipose tissue and substantially pass a second component of said unprocessed adipose tissue, such that said first filter substantially separates said first component from said second component, and wherein said first component comprises a cell population comprising adipose-derived stem cells and said second component comprises lipid and mature adipocytes; a processing chamber, which is configured to receive said first component comprising said population of cells comprising adipose-derived stem cells from said tissue collection chamber, wherein said processing chamber is within said closed system; a conduit configured to allow passage of said first component comprising said cell population comprising adipose-derived stem cells from said tissue collection chamber to said processing chamber while maintaining a closed system; a cell concentrator disposed within said processing chamber, which is configured to facilitate the concentration of said first component comprising said cell population comprising adipose-derived stem cells so as to obtain a concentrated population of cells comprising adipose-derived stem cells, wherein said cell concentrator comprises a centrifuge or a filter; and an outlet configured to allow the aseptic removal of said concentrated population of cells comprising adipose-derived stem cells; b) removing adipose tissue from the patient using the tissue removal system; c) crypreserving the adipose tissue; d) thawing the adipose tissue; e) processing the adipose tissue to obtain a concentrated population of cells comprising adipose-derived stem cells; f) inserting the device into the intended cartilage, tendon or ligament restoring area in the patient; and g) introducing the regenerative cells to the cell carrier portion of the device inserted into the patient.
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4. The method of claim 3, wherein introducing the concentrated population of cell comprising adipose-derived stem cells to said cell carrier portion of the device inserted into the patient restores cartilage, tendon or ligament in the patient.
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5. The method of claim 3, wherein the self-contained adipose-derived stem cell processing unit further comprises a programmable processing device capable of communicating with and controlling the tissue collection chamber and the cell concentrator.
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6. The method of claim 3, wherein the self contained adipose-derived stem cell processing unit further comprises a user interface for a user to input parameters into the system.
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7. The method of claim 3, wherein the self contained adipose-derived stem cell processing unit further comprises a display screen to display instructions that guide a user to input parameters, confirm pre-programmed steps, or warn of errors or combinations thereof.
Specification