CELL CARRIER AND CELL CARRIER CONTAINMENT DEVICES CONTAINING REGENERATIVE CELLS

• US 20100015204A1
• Filed: 09/28/2009
• Published: 01/21/2010
• Est. Priority Date: 12/07/2001
• Status: Abandoned Application

• Current Assignee: Lorem Vascular Pte. Ltd.
• Original Assignee: Lorem Vascular Pte. Ltd.
• Inventors: Tai, Joseph W., Fraser, John K., Hedrick, Marc H., Riley, Susan Lynn
• Application Number:
  US12/568,533
• Publication Number:
  US 20100015204A1
• Time in Patent Office: Days
• US Class Current: 424/423
• CPC Class Codes: A61F 2/442 Intervertebral or spinal discs, e.g. resilient non-disc-shaped intervertebral inflatable pockets A61F2/441; non-disc-shaped intervertebral fusion implants A61F2/4455
• CPC Class Codes: A61F 2/4455 for the fusion of spinal bodies, e.g. intervertebral fusion of adjacent spinal bodies, e.g. fusion cages intervertebral discs A61F2/442
• CPC Class Codes: A61F 2/4465 having a circular or kidney shaped cross-section substantially perpendicular to the axis of the spine
• CPC Class Codes: A61F 2/447 substantially parallelepipedal, e.g. having a rectangular or trapezoidal cross-section
• CPC Class Codes: A61F 2002/30062 (bio)absorbable, biodegradable, bioerodable, (bio)resorbable, resorptive
• CPC Class Codes: A61F 2002/30075 swellable, e.g. when wetted
• CPC Class Codes: A61F 2002/30133 kidney-shaped or bean-shaped
• CPC Class Codes: A61F 2002/30448 using adhesives
• CPC Class Codes: A61F 2002/30593 hollow
• CPC Class Codes: A61F 2002/30604 modular
• CPC Class Codes: A61F 2002/30841 Sharp anchoring protrusions for impaction into the bone, e.g. sharp pins, spikes
• CPC Class Codes: A61F 2002/30879 Ribs
• CPC Class Codes: A61F 2002/4445 Means for culturing intervertebral disc tissue
• CPC Class Codes: A61F 2002/445 Intervertebral disc tissue harvest sites
• CPC Class Codes: A61F 2210/0004 bioabsorbable
• CPC Class Codes: A61F 2210/0061 swellable
• CPC Class Codes: A61F 2220/005 using adhesives
• CPC Class Codes: A61F 2230/0015 Kidney-shaped, e.g. bean-shaped
• CPC Class Codes: A61K 35/28 Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
• CPC Class Codes: A61K 35/32 Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
• CPC Class Codes: A61K 9/0019 : Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner non-active ingredients are additionally classified in A61K47/00
• CPC Class Codes: B33Y 80/00 : Products made by additive manufacturing
• CPC Class Codes: C12N 2506/1384 : from adipose-derived stem cells [ADSC], from adipose stromal stem cells
• CPC Class Codes: C12N 5/0667 : Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
• CPC Class Codes: C12N 5/069 : Vascular Endothelial cells

Abstract

The present invention relates to a device comprising a cell carrier portion containing regenerative cells, e.g. stem and progenitor cells, and a cell carrier containment portion. The device is useful for the treatment of bone related disorders, including spinal fusion related disorders and long bone or flat bone related defects. The device may be used in conjunction with disclosed automated systems and methods for separating and concentrating regenerative cells.

Claims

1. A method of restoring cartilage, tendon or ligament in a patient, comprising:

a) providing a tissue removal system and a prosthetic device comprising a cell carrier portion and a cell carrier containment portion, said cell carrier containment portion being configured to at least partially contain the cell carrier portion;

b) removing adipose tissue from the patient using the tissue removal system;

c) cryopreserving the adipose tissue;

c) processing the cryopreserved adipose tissue to obtain a concentrated population of cells comprising adipose-derived stem cells;

d) introducing the concentrated population of cells comprising adipose-derived stem cells to the cell carrier portion of the prosthetic device; and

e) inserting the prosthetic device containing the concentrated population of cells comprising adipose-derived stem cells into the intended bone formation area in the patient.

2. The method of claim 1, wherein cartilage, tendon or ligament is restored in the patient.

3. A method of restoring cartilage, tendon or ligament in a patient comprising:

a) providing a self contained adipose derived stem cell processing unit and a device comprising a cell carrier portion and a cell carrier containment portion, wherein said cell carrier containment portion is configured to at least partially contain the cell carrier portion, wherein said self contained adipose derived stem cell processing unit comprises

a tissue collection chamber that is configured to receive unprocessed adipose tissue that is removed from said patient, wherein the tissue collection chamber is defined by a closed system;

a first filter that is disposed within said tissue collection chamber, wherein said first filter is configured to substantially retain a first component of said unprocessed adipose tissue and substantially pass a second component of said unprocessed adipose tissue, such that said first filter substantially separates said first component from said second component, and wherein said first component comprises a cell population comprising adipose-derived stem cells and said second component comprises lipid and mature adipocytes;

a processing chamber, which is configured to receive said first component comprising said population of cells comprising adipose-derived stem cells from said tissue collection chamber, wherein said processing chamber is within said closed system;

a conduit configured to allow passage of said first component comprising said cell population comprising adipose-derived stem cells from said tissue collection chamber to said processing chamber while maintaining a closed system;

a cell concentrator disposed within said processing chamber, which is configured to facilitate the concentration of said first component comprising said cell population comprising adipose-derived stem cells so as to obtain a concentrated population of cells comprising adipose-derived stem cells, wherein said cell concentrator comprises a centrifuge or a filter; and

an outlet configured to allow the aseptic removal of said concentrated population of cells comprising adipose-derived stem cells;

b) removing adipose tissue from the patient using the tissue removal system;

c) crypreserving the adipose tissue;

d) thawing the adipose tissue;

e) processing the adipose tissue to obtain a concentrated population of cells comprising adipose-derived stem cells;

f) inserting the device into the intended cartilage, tendon or ligament restoring area in the patient; and

g) introducing the regenerative cells to the cell carrier portion of the device inserted into the patient.

4. The method of claim 3, wherein introducing the concentrated population of cell comprising adipose-derived stem cells to said cell carrier portion of the device inserted into the patient restores cartilage, tendon or ligament in the patient.

5. The method of claim 3, wherein the self-contained adipose-derived stem cell processing unit further comprises a programmable processing device capable of communicating with and controlling the tissue collection chamber and the cell concentrator.

6. The method of claim 3, wherein the self contained adipose-derived stem cell processing unit further comprises a user interface for a user to input parameters into the system.

7. The method of claim 3, wherein the self contained adipose-derived stem cell processing unit further comprises a display screen to display instructions that guide a user to input parameters, confirm pre-programmed steps, or warn of errors or combinations thereof.