Systems and Methods for Improving Protein and Milk Production of Dairy Herds
First Claim
1. A method of identifying those animals having greater milk productivity from a group of livestock animals of the same species comprising:
- (a) selecting the livestock, wherein the selecting comprises;
(i) obtaining a nucleic acid molecule sample containing an ob gene polymorphism from livestock,(ii) amplifying a region of the ob gene polymorphism with the oligonucleotide pair of SEQ ID NO;
4 and SEQ ID NO;
5 to form nucleic acid amplification products,(iii) contacting the amplified ob gene polymorphism sequences from step (ii), with hybridization probes consisting essentially of the oligonucleotide pair of SEQ ID NO;
6 and SEQ ID NO;
7, labeled with a detectable moiety under suitable conditions permitting hybridization of the labeled oligonucleotide probe to amplified ob gene polymorphism sequences to form duplex structures,(iv) detecting the presence of amplified ob gene polymorphism sequences by detecting the detectable moiety of the labeled oligonucleotide probe hybridized to the amplified ob gene polymorphism sequences, and(v) selecting the type of the livestock animal based on the detection of the ob gene polymorphism; and
(b) identifying those animals having a greater milk productivity based on the presence of a particular ob gene polymorphism.
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Abstract
The present invention provides for a direct correlation between milk production in livestock animals and the presence of alleles of a gene encoding an adipocyte-specific polypeptide, termed leptin, which gene is hereinafter referred to as ob. The invention also provides novel compositions consisting essentially of specific oligonucleotides that are useful as primers to amplify particular regions of the genome during enzymatic nucleic acid amplification, thus providing a rapid, sensitive and specific method for the detection of the ob-gene polymorphism which may be present in a specimen. The invention further provides for methods of screening bovine to determine those having predictably more milk productivity and advantageously selecting those livestock for future breeding and management purposes based on the ob polymorphisms.
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Citations
22 Claims
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1. A method of identifying those animals having greater milk productivity from a group of livestock animals of the same species comprising:
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(a) selecting the livestock, wherein the selecting comprises; (i) obtaining a nucleic acid molecule sample containing an ob gene polymorphism from livestock, (ii) amplifying a region of the ob gene polymorphism with the oligonucleotide pair of SEQ ID NO;
4 and SEQ ID NO;
5 to form nucleic acid amplification products,(iii) contacting the amplified ob gene polymorphism sequences from step (ii), with hybridization probes consisting essentially of the oligonucleotide pair of SEQ ID NO;
6 and SEQ ID NO;
7, labeled with a detectable moiety under suitable conditions permitting hybridization of the labeled oligonucleotide probe to amplified ob gene polymorphism sequences to form duplex structures,(iv) detecting the presence of amplified ob gene polymorphism sequences by detecting the detectable moiety of the labeled oligonucleotide probe hybridized to the amplified ob gene polymorphism sequences, and (v) selecting the type of the livestock animal based on the detection of the ob gene polymorphism; and (b) identifying those animals having a greater milk productivity based on the presence of a particular ob gene polymorphism. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A method of increasing milk production in a selected group of livestock animals of the same species comprising:
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(a) determining a genetic predisposition of each animal to produce milk by determining their ob genotype; and (b) selecting animals that possess the T-containing allele of the ob gene for inclusion in the group. - View Dependent Claims (9, 10, 11, 20, 21, 22)
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- 12. A method of identifying those animals having increased milk productivity compared to general population of animals of the same species by determining their ob genotype wherein animals that possess the T-containing allele of the ob gene have increased milk productivity compared to animals that possess only the C-containing allele of the ob gene.
- 14. A method of breeding livestock animals to increase milk production in the offspring comprising selecting breeding pairs of livestock animals of the same species to increase occurrence of the ob T-allele in the offspring.
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16. A method of increasing milk production in a selected group of livestock animals of the same species comprising:
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(a) determining a genetic predisposition of each animal to produce milk by determining their ob genotype; (b) selecting animals that possess the T-containing allele of the ob gene for inclusion in the group; and (c) increasing the amount of feed for in the selected group. - View Dependent Claims (17, 18, 19)
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Specification