Methods and Kits for Detecting and Quantifying Damage Caused by a Parasite
First Claim
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1. A method for detecting damage caused by a parasite in a subject, the method comprising the steps of:
- a) determining the concentration of at least one biomarker in a test sample obtained from a subject, wherein the at least one biomarker is selected from the group consisting of;
choline, phosphatidylcholine, lysophosphatidyl choline and phosphocholine; and
b) comparing the concentration of the at least one biomarker determined in step (a) with a predetermined level, wherein if the concentration of the biomarker determined in step (a) is lower then the predetermined level, then the subject is determined not to have experienced damage caused by a parasite and further wherein, if the concentration of the biomarker determined in step (a) is higher then the predetermined level, then the subject is determined to have experienced damage caused by a parasite.
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Abstract
The present disclosure relates to assays and kits for detecting and quantifying damage caused by a parasite in a subject and monitoring the progression of parasitic disease in a subject.
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Citations
35 Claims
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1. A method for detecting damage caused by a parasite in a subject, the method comprising the steps of:
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a) determining the concentration of at least one biomarker in a test sample obtained from a subject, wherein the at least one biomarker is selected from the group consisting of;
choline, phosphatidylcholine, lysophosphatidyl choline and phosphocholine; andb) comparing the concentration of the at least one biomarker determined in step (a) with a predetermined level, wherein if the concentration of the biomarker determined in step (a) is lower then the predetermined level, then the subject is determined not to have experienced damage caused by a parasite and further wherein, if the concentration of the biomarker determined in step (a) is higher then the predetermined level, then the subject is determined to have experienced damage caused by a parasite. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A method for quantifying damage caused by a parasite in a subject, the method comprising the steps of:
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a) adding at least one analyte-specific enzyme to a test sample obtained from a subject to generate peroxide; b) adding at least one chemiluminescent compound to the test sample; c) measuring the light generated from the chemiluminescent compound and determining the amount of at least one biomarker present in the test sample, wherein the biomarker is selected from the group consisting of;
choline, phosphatidylcholine, lysophosphatidyl choline and phosphocholine; andd) comparing the amount of the at least one biomarker determined in step (c) with a predetermined level, wherein if the amount of the biomarker determined in step (c) is lower than the predetermined level, then the subject is determined to have a reduced severity of damage by the parasite and further wherein, if the amount of the biomarker determined in step (c) is higher then the predetermined level, then the subject is determined to have an increased severity of damage caused by the parasite. - View Dependent Claims (10, 11, 12, 13, 14, 15)
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16. A method for monitoring progression of damage caused by parasitic disease in a subject, the method comprising the steps of:
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a) determining the concentration at least one biomarker in a test sample obtained from a subject, wherein the at least one biomarker is selected from the group consisting of;
choline, phosphatidylcholine, lysophosphatidyl choline and phosphocholine; andb) comparing the concentration of the at least one biomarker determined in step (a) with a predetermined level, wherein if the concentration of the biomarker determined in step (a) is lower than the predetermined level, then the damage caused by the parasitic disease in the subject is determined not to have progressed or to have improved and further wherein if the concentration of the biomarker determined in step (a) is higher then the predetermined level, then the damage caused by the parasitic disease in the subject is determined not to have progressed. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23)
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24. A kit for use in determining the presence or amount of damage caused by a parasite in a subject, the kit comprising:
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a. at least one analyte specific enzyme; b. at least one chemiluminescent compound; and c. instructions for detecting the presence or amount of damage caused by a parasite in a test sample. - View Dependent Claims (25, 26, 27, 28, 29)
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30. A kit for monitoring parasitic disease in a subject, the kit comprising:
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a. at least one analyte specific enzyme; b. at least one chemiluminescent compound; and c. instructions for monitoring parasitic disease in an individual. - View Dependent Claims (31, 32, 33, 34, 35)
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Specification