COMPONENTS OF CELL CULTURE MEDIA PRODUCED FROM PLANT CELLS

• US 20100015713A1
• Filed: 06/27/2006
• Published: 01/21/2010
• Est. Priority Date: 06/28/2005
• Status: Abandoned Application

• Current Assignee: Invitria Incorporated (Ventria Bioscience Company)
• Original Assignee: Invitria Incorporated (Ventria Bioscience Company)
• Inventors: Deeter, Scott, Bethell, Delia R., Mabery, Kenneth J., Schmidt, Joseph E., Pettit, Steve Clyde, Huang, Ning
• Application Number:
  US11/922,984
• Publication Number:
  US 20100015713A1
• Time in Patent Office: Days
• US Class Current: 435/406
• CPC Class Codes: C12N 15/8257 for the production of primary gene products, e.g. pharmaceutical products, interferon
• CPC Class Codes: C12N 2501/998 Proteins not provided for elsewhere
• CPC Class Codes: C12N 2510/02 Cells for production
• CPC Class Codes: C12N 5/0018 Culture media for cell or tissue culture media for specific animal cell type C12N5/06

Abstract

The present invention comprises the production of components of cell culture media produced from plant cells and cell culture media containing the same. Heterologous DNA comprising genes encoding the desired component are introduced into plant cells, especially rice, which then produce the desired component. The component can be isolated from the plant cell and combined with other components to form the required cell culture medium.

Claims

1. A cell culture media, comprising at least one plant-produced heterologous protein as a cell culture media component.

2. The cell culture media of claim 1, wherein the plant is a monocot plant.

3. The cell culture media of claim 2, wherein the monocot plant is rice, barley, wheat, rye, corn, millet, triticale, or sorghum.

4. The cell culture media of claim 3, wherein the monocot plant is rice.

5. The cell culture media of claim 1, wherein the plant is a dicot plant.

6. The cell culture media of claim 1, wherein the heterologous protein is a plant protein.

7. The cell culture media of claim 1, wherein the heterologous protein is a non-plant protein.

8. The cell culture media of claim 7, wherein the non-plant protein is an animal protein.

9. The cell culture media of claim 7, wherein the non-plant protein is a human protein.

10. The cell culture media of claim 1, wherein the protein is selected from the group consisting of growth factors, lactoferrin, transferrin, serum albumin, insulin, growth hormone, and fractions thereof, fibronectin attachment factor, lamin attachment factor, collagenase, platelet derived growth factor, brain-derived neutrophic factor, glial-derived neurotrophic factor, thymic factors, haptocorin, lactahedrin, lactoperoxidase, alpha-fetoprotein, immunoglobin, or alpha-lactalbumin.

11. The cell culture media of claim 10, wherein the growth factors are epidermal growth factors, keratinocyte growth factors, insulin-like growth factors, intestinal trefoil factors, transforming growth factors, granulocyte colony-stimulating factors, nerve growth factors, fibroblast growth factors, or biologically active fragments thereof.

12. The cell culture media of claim 1, wherein the cell culture media is a reduced serum, serum-free medium, or chemically defined media.

13. The cell culture media of claim 1, wherein the cell culture media is a complete media, basal media, or basal media supplemented with a cell culture media component.

14. A method for producing an enhanced cell culture media, comprising producing at least one heterologous protein in a plant cell and combining the at least one plant-produced heterologous protein with a cell culture media.

15. A cell culture media produced by a process comprising producing at least one heterologous protein in a plant cell and combining the at least one plant-produced heterologous protein with a cell culture media.

16. In a cell culture media having one or more proteinaceous cell culture media components, the improvement comprising at least one plant-produced heterologous protein as a cell culture media component, wherein the heterologous protein is produced by the following process:

a) transforming a plant cell with a chimeric gene comprising;

(i) a promoter from the gene of a seed storage protein;

(ii) a first DNA sequence, operably linked to the promoter, encoding a signal sequence of a seed storage protein, and

(iii) a second DNA sequence, operably linked to the promoter, encoding the heterologous protein, wherein the first and second DNA sequences are linked in translation frame and together encode a fusion protein comprising the storage protein and the heterologous protein; and

b) growing a plant from the transformed plant cell for a time sufficient to produce seeds containing the heterologous protein.

17. The method of claim 17, further comprising purifying the heterologous protein from the harvested seeds.

18. The method of claim 17, wherein the heterologous protein constitutes at least about 3.0% of the total soluble protein in the harvested seeds.

19. A method for producing a cell culture media having at least one plant-produced heterologous protein as a cell culture media component, the method comprising:

a) transforming a plant cell with a chimeric gene comprising;

(i) a promoter from the gene of a seed storage protein;

(ii) a first DNA sequence, operably linked to the promoter, encoding a signal sequence of a seed storage protein, and

(iii) a second DNA sequence, operably linked to the promoter, encoding the heterologous protein, wherein the first and second DNA sequences are linked in translation frame and together encode a fusion protein comprising the storage protein and the heterologous protein;

b) growing a plant from the transformed plant cell for a time sufficient to produce seeds containing the heterologous protein;

c) harvesting the seeds from the plant; and

d) combining the heterologous protein with a cell culture media.