DEVICE FOR MEASURING CELLULAR POTENTIAL, SUBSTRATE USED FOR THE SAME AND METHOD OF MANUFACTURING SUBSTRATE FOR DEVICE FOR MEASURING CELLULAR POTENTIAL

• US 20100019756A1
• Filed: 05/11/2007
• Published: 01/28/2010
• Est. Priority Date: 05/17/2006
• Status: Abandoned Application

• Current Assignee: Panasonic Corporation (Panasonic Holdings Corporation)
• Original Assignee: Matsushita Electric Industrial Company Limited (Panasonic Holdings Corporation)
• Inventors: Ushio, Hiroshi, Nakatani, Masaya, Hiraoka, Soichiro
• Application Number:
  US11/913,116
• Publication Number:
  US 20100019756A1
• Time in Patent Office: Days
• US Class Current: 324/71.100
• CPC Class Codes: G01N 33/48728 Investigating individual cells, e.g. by patch clamp, voltage clamp investigating individual particles in general G01N15/10
• CPC Class Codes: G01N 33/5005 involving human or animal cells immunoassay G01N33/56966; immunoassays of protozoa G01N33/56905; protozoa in screening assays C12Q1/025
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: Y10T 428/24273 including aperture
• CPC Class Codes: Y10T 428/24298 Noncircular aperture [e.g., slit, diamond, rectangular, etc.]

Abstract

A device for measuring cellular potential includes a substrate, a first electrode tank, a first electrode, a second electrode tank and a second electrode. The first electrode tank and the second electrode tank are disposed on the upper side and lower side of the substrate, respectively. The first electrode is disposed inside the first electrode tank and the second electrode is disposed inside the second electrode tank. The substrate includes a single crystal plate having a diamond structure with (100) plane orientation or (110) plane orientation. The substrate has a first surface provided with a depression and a second surface facing this first surface. From the depression to the second surface, a through-hole is formed. The depression has an inner wall extending from the opening of the through-hole to the outer periphery, curving and being connected to the first surface.

Claims

1. A substrate for a device for measuring cellular potential, comprising:

a single crystal plate including a first surface and a second surface facing the first surface, and having a diamond structure with (100) plane orientation;

wherein the first surface is provided with a depression, a through-hole is formed from the depression to the second surface, and the depression has an inner wall extending from an opening of the through-hole to an outer periphery, curving and being connected to the first surface.

2. The substrate according to claim 1, wherein the depression has a hemispherical shape.

3. The substrate according to claim 1, wherein a diameter of the through-hole is more than 0 μ

m and not more than 3 μ

m.

4. The substrate according to claim 1, further comprising a film made of an insulating material disposed on a surface of the depression.

5. The substrate according to claim 1, wherein a surface roughness of an inner wall of the through-hole is larger than a surface roughness of the inner wall of the depression.

6. The substrate according to claim 1, further comprising an etching stop layer that is formed on the second surface of the substrate and has an etching rate smaller than an etching rate of a material constituting the substrate.

7. A device for measuring cellular potential, comprising:

a substrate for a device for measuring cellular potential according to claim 1,

a first electrode tank disposed on an upper side of the substrate;

a first electrode disposed inside the first electrode tank;

a second electrode tank disposed on a lower side of the substrate; and

a second electrode disposed inside the second electrode tank.

8. A substrate for a device for measuring cellular potential, comprising:

a single crystal plate including a first surface and a second surface facing the first surface, and having a diamond structure with (110) plane orientation;

wherein the first surface is provided with a depression, a through-hole is formed from the depression to the second surface, and the depression has an inner wall extending from an opening of the through-hole to an outer periphery, curving and being connected to the first surface.

9. The substrate according to claim 8, wherein the depression has a semi-elliptical sphere shape.

10. The substrate according to claim 8, wherein a diameter of the through-hole is more than 0 μ

m and not more than 3 μ

m.

11. The substrate according to claim 8, further comprising a film made of an insulating material disposed on a surface of the depression.

12. The substrate according to claim 8, wherein a surface roughness of an inner wall of the through-hole is larger than a surface roughness of the inner wall of the depression.

13. The substrate according to claim 8, further comprising an etching stop layer that is formed on the second surface of the substrate and has an etching rate smaller than an etching rate of a material constituting the substrate.

14. A device for measuring cellular potential, comprising:

a substrate for a device for measuring cellular potential according to claim 8,

a first electrode tank disposed on an upper side of the substrate;

a first electrode disposed inside the first electrode tank;

a second electrode tank disposed on a lower side of the substrate; and

a second electrode disposed inside the second electrode tank.

15. A method of manufacturing a substrate for a device for measuring cellular potential, the method comprising:

forming a resist mask having a mask hole on a first surface of a single crystal plate, the single crystal plate including the first surface and a second surface facing the first surface and having any one of a diamond structure with (100) plane orientation and a diamond structure with (110) plane orientation by using a photo mask;

forming a depression on the first surface by dry etching; and

providing a through-hole having a same diameter as that of the mask hole, the through-hole being allowed to penetrate the single crystal plate from the depression to the second surface by dry etching.

16. The method according to claim 15, wherein an opening diameter of the mask hole is more than 0 μ

m and not more than 3 μ

m.

17. The method according to claim 15, wherein in the forming of the depression, an etching gas is used, and the etching gas is at least one of CF₄, SF₆, NF₃ and XeF₂.

18. The method according to claim 15, wherein in the forming of the depression, an etching gas and a carrier gas are used, and the carrier gas is at least one of N₂, Ar, He and H₂.

19. The method according to claim 15, wherein in the forming of the depression, an etching gas and a carrier gas are used, and a molar ratio of the etching gas to the carrier gas is more than 0 and not more than 2.0.

20. The method according to claim 15, wherein in the forming of the depression, an etching gas is used, and filling and removing of the etching gas are repeated a plurality of times.

21. The method according to claim 15, wherein in the providing of the through-hole, an etching gas and an etching suppressing gas are used alternately.

22. The method according to claim 21, wherein the etching gas is at least one of CF₄, SF₆, NF₃ and XeF₂.

23. The method according to claim 21, wherein the etching suppressing gas is at least one of CHF₃ and C₄F₈.