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3-Color multiplex cars spectrometer

  • US 20100020318A1
  • Filed: 12/29/2008
  • Published: 01/28/2010
  • Est. Priority Date: 07/24/2008
  • Status: Active Grant
First Claim
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1. A 3-color multiplex CARS spectrometer, comprising:

  • a broadband laser light source 10 generating a pump beam for multiply exciting molecular vibrations of media constituting a sample;

    a Stokes laser light source 20 generating a Stokes beam for allowing the multiply excited molecular vibration to resonate while interacting with the pump beam;

    a probe laser light source 30 generating a probe beam for generating a multiplex CARS spectrum signal from the resonance-excited molecular vibration of the sample;

    a beam combiner 40 temporally synchronizing optical pulse outputs of the pump, Stokes and probe beams, and spatially overlapping the pump, Stokes and probe beams on the same axis;

    a microscope objective 50 focusing the synchronized and spatially overlapped laser beam onto a point in the sample;

    a sample displacement scanner 60 arbitrarily adjusting the position of a focus of the laser beam in the sample focused by the microscope objective 50;

    a condensing optical system 70 condensing and collimating a CARS signal generated at the focus of the sample;

    a dichroic mirror 80 or notch filter removing the incident laser and noise wavelength components contained in the condensed CARS signal;

    a monochromator 90 spatially dispersing wavelength components of the pure CARS signal from which the incident laser and noise wavelength components are removed;

    a 1D/2D photodetector array 100 detecting a multiplex CARS spectral signal wavelength-dispersed by the monochromator 90; and

    a data analyzer 110 transferring the wavelength-dispersed multiplex CARS spectral signal detected by the 1D/2D photodetector array 100 to a computer and performing data analysis.

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