MULTIPOTENT ADULT STEM CELL DERIVED FROM CANINE UMBILICAL CORD BLOOD, PLACENTA AND CANINE FETUS HEART, METHOD FOR PREPARING THE SAME AND CELLULAR THERAPEUTICS CONTAINING THE SAME
First Claim
1. A method for producing an adult stem cell, wherein the adult stem cell is obtained by culturing eukaryotic cells derived from blood sample from canine fetal heart, and canine umbilical cord blood or placental blood, in FBS-containing medium and show the following characteristics of:
- (a) showing positive immunological responses to one or more of antigens selected from the group consisting of MHC class I, CD44(BD) and CD90, and positive or negative immunological responses to CD34, and negative immunological responses to CD45, CD14, CD3, CD4, CD8, CD11c, CD 172a and HLA-DR;
(b) growing adhered to plastic and showing spindle-shaped morphological features; and
(c) having the ability to differentiate into the cells derived from endoderm, ectoderm, and mesoderm.
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Accused Products
Abstract
The present invention relates to multipotent adult stem cells derived from canine umbilical cord blood, placental blood and blood sample from canine fetal heart, and a method for preparing the same as well as a cellular therapeutic agent containing the same, more specifically, to a multipotent adult stem cell isolated by culturing an eukaryotic cell derived from canine umbilical cord blood, placental blood and blood sample from canine fetal heart in a FBS-containing medium and a method for preparing the same. Adult stem cells according to the present invention are derived from canine umbilical cord blood, placental blood and blood sample from canine fetal heart. The adult stem cells have characteristics highly similar to human mesenchymal stem cells as well as remarkable cell growth at the initial step compared to human UCB-derived mesenchymal stem cells so that the cells are useful to treat canine incurable diseases and difficult-to-cure diseases. Furthermore, multipotent adult stem cells are effective to treat musculoskeletal diseases and neural diseases due to the ability to differentiate into osteogenic cells and neural cells.
5 Citations
15 Claims
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1. A method for producing an adult stem cell, wherein the adult stem cell is obtained by culturing eukaryotic cells derived from blood sample from canine fetal heart, and canine umbilical cord blood or placental blood, in FBS-containing medium and show the following characteristics of:
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(a) showing positive immunological responses to one or more of antigens selected from the group consisting of MHC class I, CD44(BD) and CD90, and positive or negative immunological responses to CD34, and negative immunological responses to CD45, CD14, CD3, CD4, CD8, CD11c, CD 172a and HLA-DR; (b) growing adhered to plastic and showing spindle-shaped morphological features; and (c) having the ability to differentiate into the cells derived from endoderm, ectoderm, and mesoderm. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification