ADDRESSABLE ANTIBODY ARRAYS AND METHODS OF USE
First Claim
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1. An assay method for detecting an autoantibody in a sample from a subject, said method comprising:
- (a) contacting a tagged antigen with a sample having an autoantibody specific for said tagged antigen to transform said autoantibody into an immunological pair;
(b) contacting said immunological pair with a solid support having a binding member specific for said immunological pair to transform said immunological pair into a protein complex;
(c) separating said protein complex from said sample to form an isolated protein complex; and
(d) releasing said tag from said isolated protein complex for detection.
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Abstract
Systems and assay methods are disclosed for detecting an autoantibody in a sample. In certain instances, the systems and methods employ a mass tag releasably connected to an antigen. The tag is thereafter released for detection. A tag can be detected by mass spectrometry or in certain instances the tag is fluorescent. Methods for diagnosing a disease or disorder in a subject are also disclosed.
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Citations
21 Claims
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1. An assay method for detecting an autoantibody in a sample from a subject, said method comprising:
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(a) contacting a tagged antigen with a sample having an autoantibody specific for said tagged antigen to transform said autoantibody into an immunological pair; (b) contacting said immunological pair with a solid support having a binding member specific for said immunological pair to transform said immunological pair into a protein complex; (c) separating said protein complex from said sample to form an isolated protein complex; and (d) releasing said tag from said isolated protein complex for detection. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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20. A method for diagnosing a disease or disorder in a subject, said method comprising:
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(a) contacting a tagged antigen with a sample from said subject having an autoantibody specific for said tagged antigen to transform said tagged antigen into an immunological pair; (b) contacting said immunological pair with a solid support having a binding member specific for said immunological pair to transform said immunological pair into a protein complex; (c) separating said protein complex from said sample to form an isolated protein complex; (d) releasing said tag from said isolated protein complex for detection; and (e) detecting the amount of said tag, wherein the amount of said tag is indicative of the amount of said autoantibody, and wherein said disease or disorder is determined to be present when the amount of said autoantibody differs from a control value representing the amount of said autoantibody in a sample from a subject not having said disease or disorder.
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21. An assay method for detecting an autoantibody in a sample from a subject, said method comprising:
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(a) contacting a tagged antigen with a sample having an autoantibody specific for said tagged antigen to transform said tagged antigen into an immunological pair; (b) contacting said immunological pair with a solid support having a binding member specific for said immunological pair to transform said immunological pair into a protein complex; (c) releasing said protein complex to form an isolated protein complex; and (d) recapturing said isolated protein complex.
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Specification