PLATELET AGGREGATION INDUCING SUBSTANCE

• US 20100022755A1
• Filed: 12/12/2007
• Published: 01/28/2010
• Est. Priority Date: 12/21/2006
• Status: Active Grant

• Current Assignee: JNC Corporation (Chisso Corporation)
• Original Assignee: Chisso Corporation
• Inventors: Takasaki, Shinichi, Kawai, Takahiro, Umeda, Yasuto, Takebayashi, Takafumi
• Granted Patent: US 8,686,117 B2
• Time in Patent Office: Days
• US Class Current: 530/381
• CPC Class Codes: C07K 14/001 by chemical synthesis
• CPC Class Codes: C07K 14/78 Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
• CPC Class Codes: Y10T 428/2982 Particulate matter [e.g., sphere, flake, etc.]

Abstract

A platelet aggregation inducing substance containing as an active ingredient a polypeptide having a peptide fragment represented by formula (1) (component A):

-(Pro-X-Gly)_n-   (1)

wherein X represents Pro or Hyp; and n represents an integer of from 20 to 5,000.

Claims

1. A platelet aggregation inducing substance comprising as an active ingredient a polypeptide having a peptide fragment represented by formula (1) (component A):

-(Pro-X-Gly)_n-  

 

(1)

wherein X represents Pro or Hyp; and

n represents an integer of from 20 to 5,000.

2. The platelet aggregation inducing substance according to claim 1, wherein the peptide fragment (component A) comprises a peptide unit represented by formula (2) (component B) and a peptide unit represented by formula (3) (component C), and a ratio of the component B and the component C (component B/component C, molar ratio) is in a range of from 95/5 to 0/100:

-(Pro-Pro-Gly)-  

 

(2)

-(Pro-Hyp-Gly)-  

 

(3)

3. The platelet aggregation inducing substance according to claim 1, wherein the polypeptide further comprises at least one selected from a peptide unit represented by formula (4), a peptide unit represented by formula (5), and a peptide fragment containing at least one of the peptide units (component D):

—

(Y₁—

Y₂-Gly)-  

 

(4)

-(Pro-Z₁-Gly-Z₂-Ala-Gly)-  

 

(5)

wherein Y₁ represents Asp or Glu, which may have a carboxyl group at the γ

-position;

Y₂ represents Pro or Hyp;

Z₁ represents Gln, Asn, Leu, Ile, Val or Ala; and

Z₂ represents Ile or Leu.

4. The platelet aggregation inducing substance according to claim 3, wherein a ratio of the peptide fragment (component A) and at least one selected from the peptide unit represented by formula (4), the peptide unit represented by formula (5), and the peptide fragment containing at least one of the peptide units (component D) (component A/component D, molar ratio) is in a range of from 99/1 to 30/70.

5. The platelet aggregation inducing substance according to claim 1, wherein the polypeptide exhibits positive Cotton effect at a wavelength of from 220 to 230 nm and negative Cotton effect at a wavelength of from 195 to 205 nm in a circular dichroic spectrum.

6. The platelet aggregation inducing substance according to claim 1, wherein the polypeptide has a molecular weight in a range of from 10,000 to 500,000,000.

7. The platelet aggregation inducing substance according to claim 1, wherein the polypeptide has a particle diameter in a range of from 0.01 to 1,000 μ

m.

8. The platelet aggregation inducing substance according to claim 1, wherein the polypeptide has a viscosity in a range of from 10 to 10,000 mPa·

s, wherein the viscosity is measured for a 1 wt % aqueous solution of the polypeptide under a condition of 20°

C. with an E-type viscometer.

9. The platelet aggregation inducing substance according to claim 2, wherein the polypeptide further comprises at least one selected from a peptide unit represented by formula (4), a peptide unit represented by formula (5), and a peptide fragment containing at least one of the peptide units (component D):

—

(Y₁—

Y₂-Gly)-  

 

(4)

-(Pro-Z₁-Gly-Z₂-Ala-Gly)-  

 

(5)

wherein Y₁ represents Asp or Glu, which may have a carboxyl group at the γ

-position;

Y₂ represents Pro or Hyp;

Z₁ represents Gln, Asn, Leu, Ile, Val or Ala; and

Z₂ represents Ile or Leu.

10. The platelet aggregation inducing substance according to claim 9, wherein a ratio of the peptide fragment (component A) and at least one selected from the peptide unit represented by formula (4), the peptide unit represented by formula (5), and the peptide fragment containing at least one of the peptide units (component D) (component A/component D, molar ratio) is in a range of from 99/1 to 30/70.