Potentiometric biosensor and the forming method thereof
First Claim
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1. A potentiometric biosensor, comprising:
- a substrate;
at least two working electrodes formed on said substrate;
at least one counter electrode formed on said substrate;
an internal reference electrode formed on said substrate; and
a packaging structure, which separates the adjacent electrodes.
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Abstract
The present invention discloses a potentiometric biosensor for urea and creatinine detection, and the forming method thereof. The disclosed biosensor comprises a substrate, at least two working electrode on the substrate, at least one reference electrode on the substrate, an internal reference electrode on the substrate, and a packaging structure which separates the adjacent electrodes. The working electrode comprises urease or creatinine iminohydrolase (CIH). The detection signal is transmitted for further processing through a wire or an exposed surface on the biosensor. The disclosed biosensor is replaceable.
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Citations
30 Claims
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1. A potentiometric biosensor, comprising:
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a substrate; at least two working electrodes formed on said substrate; at least one counter electrode formed on said substrate; an internal reference electrode formed on said substrate; and a packaging structure, which separates the adjacent electrodes. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 28)
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25. A method of forming a potentiometric biosensor, comprising:
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providing a substrate; forming an internal reference electrode on said substrate; forming at least one counter electrode on said substrate; forming at least two working electrodes on said substrate; and forming a packaging structure to separate the adjacent electrodes. - View Dependent Claims (26, 27)
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29. A method of measuring a potentiometric biosensor, comprising:
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measuring a reference voltage via putting at least two working electrode into a buffer solution; amplifying a readout signal of at least two working electrodes using at least two instrumental amplifiers; and measuring a reaction voltage via putting at least two working electrodes into a tested solution. - View Dependent Claims (30)
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Specification