Amnion-Derived Cell Compositions, Methods of Making and Uses Thereof
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Abstract
The invention is directed to substantially purified amnion-derived cell populations, compositions comprising the substantially purified amnion-derived cell populations, and to methods of creating such substantially purified amnion-derived cell populations, as well as methods of use. The invention is further directed to antibodies, in particular, monoclonal antibodies, that bind to amnion-derived cells or, alternatively, to one or more amnion-derived cell surface protein markers. The invention is further directed to methods for producing the antibodies, methods for using the antibodies, and kits comprising the antibodies.
29 Citations
49 Claims
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- 17. A population of early pancreatic progenitor cells differentiated from a substantially purified population of amnion-derived cells that is negative for the expression of the protein markers CD90 and CD117, wherein the early pancreatic progenitor cells express a pancreatic progenitor cell marker protein.
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- 42. An islet progenitor cell population differentiated from amnion-derived cells that are negative for the expression of the protein markers CD90 and CD117, wherein the islet progenitor cell population expresses at least one early pancreatic determination gene.
- 44. A cell population differentiated from amnion-derived cells that are negative for the expression of the protein markers CD90 and CD117, wherein the cell population has the identifying characteristics of endoderm.
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49. An in vivo method for promoting the differentiation of amnion-derived cells into pancreatic cells comprising
(a) introducing factors to the population of amnion-derived cells in vitro; - and
(b) subsequently transplanting the amnion-derived cells into the pancreas of a subject.
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Specification