METHOD FOR EVALUATION OF TISSUE PRESERVATION SOLUTION

• US 20100028883A1
• Filed: 01/18/2008
• Published: 02/04/2010
• Est. Priority Date: 03/13/2007
• Status: Active Grant

• Current Assignee: Jichi Medical University, Otsuka Pharmaceutical Factory Incorporated (Otsuka Holdings Company Limited)
• Original Assignee: Jichi Medical University, Otsuka Pharmaceutical Factory Incorporated (Otsuka Holdings Company Limited)
• Inventors: Kobayashi, Eiji
• Granted Patent: US 8,871,440 B2
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12Q 1/66 involving luciferase
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: G01N 33/5082 Supracellular entities, e.g. tissue, organisms

Abstract

The present invention provides a method for evaluating preservative effect of a tissue preservation solution, comprising immersing a mammalian tissue introduced with a luminescence or fluorescence labeling gene in the tissue preservation solution, measuring a luminescence or fluorescence level by the labeling gene in the tissue after immersion, and evaluating the preservative effect of the tissue preservation solution based on the luminescence or fluorescence level.

Claims

1. A method for evaluating preservative effect of a tissue preservation solution, comprising immersing a mammalian tissue introduced with a luminescence or fluorescence labeling gene in the tissue preservation solution, measuring a luminescence or fluorescence level by the labeling gene in the tissue after immersion, and evaluating the preservative effect of the tissue preservation solution based on the luminescence or fluorescence level.

2. The method of claim 1, wherein the labeling gene is luciferase.

3. The method of claim 1, wherein the luminescence or fluorescence level in the tissue is measured nondisruptively.

4. The method of claim 1, wherein the tissue is isolated from a non-human mammal introduced with the luminescence or fluorescence labeling gene.

5. The method of claim 4, wherein the labeling gene is expressed ubiquitously in the mammal.

6. The method of claim 4, wherein the target gene is specifically expressed in the object tissue of the mammal.

7. The method of claim 1, wherein the tissue preservation solution is a cell preservation solution.

8. The method of claim 1, wherein the tissue preservation solution is an organ preservation solution.

9. The method of claim 1, wherein the mammalian tissue is a part of an organ.

11. The method of claim 2, wherein the tissue preservation solution is a cell preservation solution.

12. The method of claim 3, wherein the tissue preservation solution is a cell preservation solution.

13. The method of claim 4, wherein the tissue preservation solution is a cell preservation solution.

14. The method of claim 5, wherein the tissue preservation solution is a cell preservation solution.

15. The method of claim 6, wherein the tissue preservation solution is a cell preservation solution.

16. The method of claim 2, wherein the tissue preservation solution is an organ preservation solution.

17. The method of claim 3, wherein the tissue preservation solution is an organ preservation solution.

18. The method of claim 4, wherein the tissue preservation solution is an organ preservation solution.

19. The method of claim 5, wherein the tissue preservation solution is an organ preservation solution.

20. The method of claim 6, wherein the tissue preservation solution is an organ preservation solution.

10. A method of producing a tissue preservation solution having a confirmed preservative effect, comprising the following steps:

(I) mixing constituent components of a desired tissue preservation solution to give the tissue preservation solution;

(II) separating a part of the tissue preservation solution obtained in (I) as a sample;

(III) immersing a mammalian tissue introduced with a luminescence or fluorescence labeling gene in the sample separated in (II);

(IV) measuring the level of luminescence or fluorescence by the labeling gene in the tissue after immersion;

(V) evaluating the preservative effect of the sample based on the luminescence or fluorescence level; and

(VI) obtaining, as a tissue preservation solution with confirmed preservative effect, the tissue preservation solution from which the sample confirmed to have the desired preservative effect in (V) derives.