Labelled nucleotides
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Accused Products
Abstract
Nucleosides and nucleotides are disclosed that are linked to detectable labels via a cleavable linker group.
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Citations
35 Claims
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1-16. -16. (canceled)
- 17. A method for determining the sequence of an immobilized target polynucleotide, comprising (a) monitoring the sequential incorporation of nucleotides complementary to the immobilized target polynucleotide, wherein the nucleotides each have a base that is linked to a detectable label via a cleavable linker, and wherein the detectable label linked to each of the nucleotides is distinguished upon detection from the detectable label used for other types of nucleotides and wherein the identity of each nucleotide incorporated is determined by detection of the label linked to the base, and (b) removing the label from the base.
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18. A method for determining the sequence of an immobilized target polynucleotide, comprising:
- (a) providing nucleotides, wherein the nucleotides have a base that is linked to a detectable label via a cleavable linker, and wherein the detectable label linked to each type of nucleotide can be distinguished upon detection from the detectable label used for other types of nucleotides;
(b) incorporating a nucleotide into the complement of the immobilized target polynucleotide;
(c) detecting the label of the nucleotide incorporated in (b), thereby determining the type of nucleotide incorporated;
(d) removing the label of the nucleotide detected in (c); and
(e) repeating steps (b)-(d) one or more times;
thereby determining the sequence of a target polynucleotide.
- (a) providing nucleotides, wherein the nucleotides have a base that is linked to a detectable label via a cleavable linker, and wherein the detectable label linked to each type of nucleotide can be distinguished upon detection from the detectable label used for other types of nucleotides;
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19. (canceled)
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24. (canceled)
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25. (canceled)
Specification