Artificial Protein Scaffolds
First Claim
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1. A protein comprising a Top7 fold, wherein one or more loops in the Top7 fold bind specifically to a preselected target molecule, wherein the protein binds to the preselected target molecule with a dissocation constant of no more than 10 μ
- M.
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Abstract
The present invention provides proteins having one or more similarities to the artificial protein Top7 or to a Top7 derivative. Proteins of the invention have one or more loops that are longer than the corresponding loops of Top7, and/or that bind to a preselected target molecule. The invention also provides nucleic acids and cells useful in producing the proteins and methods for their use.
9 Citations
56 Claims
- 1. A protein comprising a Top7 fold, wherein one or more loops in the Top7 fold bind specifically to a preselected target molecule, wherein the protein binds to the preselected target molecule with a dissocation constant of no more than 10 μ
- 2. A protein comprising a Top7 fold that defines two ends, wherein at least two loops on one end of the protein are each at least one amino acid longer than the corresponding loops of Top7.
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3-23. -23. (canceled)
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24. A protein comprising an amino acid sequence of the formula B(1)-L(12)-B(2)-L(23)-A(3)-L(34)-B(4)-L(45)-A(5)-L(56)-B(6)-L(67)-B(7),
wherein B(1), B(2), A(3), B(4), A(5), B(6), and B(7) correspond to amino acids 1-5, 6-8, 9-20, 21-23, 24-32, 33-37, and 38-42 of (i) an amino acid sequence at least 85% identical to SEQ ID NO: - 3;
or (ii) a sequence at least 95% identical to SEQ ID NO;
6;
or (iii) a sequence identical to SEQ ID NO;
7,wherein the minimum length of L(12) is 10 amino acids, wherein the minimum length of L(23) is 7 amino acids, wherein the minimum length of L(34) is 9 amino acids, wherein the minimum length of L(45) is 10 amino acids, wherein the minimum length of L(56) is 7 amino acids, wherein the minimum length of L(67) is 4 amino acids, and wherein L(12), L(23), L(34), L(45), L(56), or L(67) exceeds its minimum length by at least one amino acid.
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25-37. -37. (canceled)
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45. (canceled)
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46. (canceled)
Specification