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PREVENTION AND ALLEVIATION OF STERIC HINDRANCE DURING SINGLE MOLECULE SYNTHESIS

  • US 20100035269A1
  • Filed: 08/04/2009
  • Published: 02/11/2010
  • Est. Priority Date: 08/05/2008
  • Status: Active Grant
First Claim
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1. A method for reducing steric hindrance resulting from a product of a nucleic acid polymerase reaction, said method comprising conducting said nucleic acid polymerase reaction in the presence of an enzyme selected from the group consisting of an exonuclease, an endonuclease, and a uracil-DNA glycosylase, wherein said nucleic acid polymerase reaction includes a template nucleic acid, and wherein said enzyme modifies said product but does not modify said template nucleic acid.

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