SYSTEMS, DEVICES, AND, METHODS FOR RELEASING BIOMASS CELL COMPONENTS

• US 20100035321A1
• Filed: 04/16/2008
• Published: 02/11/2010
• Est. Priority Date: 04/20/2007
• Status: Abandoned Application

• Current Assignee: Bionavitas, Inc.
• Original Assignee: Bionavitas, Inc.
• Inventors: Wilkerson, Brian D., Burns, James A. JR., Chen, James C.
• Application Number:
  US12/104,172
• Publication Number:
  US 20100035321A1
• Time in Patent Office: Days
• US Class Current: 435/173.100
• CPC Class Codes: C12M 21/02 Photobioreactors culturing algae A01G33/00, A01H4/001, C12N1/12
• CPC Class Codes: C12M 31/10 by light emitting elements located inside the reactor, e.g. LED or OLED
• CPC Class Codes: C12M 33/00 Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus chemical or physical laboratory apparatus in general B01L, devices for taking cell samples A61B10/0045, withdrawing or distributing predetermined quantities of fluid B01L99/00
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
• CPC Class Codes: C12N 5/04 Plant cells or tissues culture media C12N5/0025
• CPC Class Codes: C12P 1/00 Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
• CPC Class Codes: C12P 7/6409 Fatty acids
• CPC Class Codes: C12P 7/6463 obtained from glyceride producing microorganisms, e.g. single cell oil

Abstract

Systems, devices, and methods for releasing one or more cell components from a photosynthetic organism. A bioreactor system is operable for growing photosynthetic organisms. Some of the methods include contacting the photosynthetic organism with an energy-activatable sensitizer, and activating the energy-activatable sensitizer, thereby releasing a cellular component from at least one of, for example, a membrane structure, tubule, vesicle, cisterna, organelle, cell compartment, plastid, or mitochondrion, associated with the photosynthetic organisms.

Claims

1. A method for releasing a cell component from a photosynthetic organism, comprising:

contacting the photosynthetic organism with an energy-activatable sensitizer; and

activating the energy-activatable sensitizer, thereby releasing a cellular component from at least one of a membrane structure, tubule, vesicle, cisterna, organelle, cell compartment, plastid, or mitochondrion, associated with the photosynthetic organisms.

2. The method of claim 1, wherein said energy-activatable sensitizer is activatable by absorption of light, sonic, ultrasonic, thermal, and/or chemical energy.

3. The method of claim 1, wherein said cellular components are recovered.

4. The method of claim 1, further comprising:

providing a permeabilizer to the photosynthetic organism, the permeabilizer capable of promoting absorption of the energy-activatable photosensitizer by the photosynthetic organism.

5. The method of claim 4, wherein the permeabilizer comprises polyethylenimine.

6. The method of claim 1 wherein the energy activatable photosensitizer is activatable by absorption of energy having a wavelength in the visible, ultra violet, and/or infrared range.

7. The method of claim 1 wherein the energy activatable photosensitizer is activatable by absorption of energy having a wavelength ranging from about 500 nm to about 1100 nm.

8. The method of claim 1 wherein the energy activatable photosensitizer is activatable by absorption of energy having a wavelength ranging from about 200 nm to about 400 nm.

9. The method of claim 1 wherein the energy activatable photosensitizer is activatable by absorption of energy having a wavelength ranging from about 400 nm to about 780 nm.

10. The method of claim 1 wherein the released cellular components comprise one or more growth factors, amino acids, carotenoids, bioflavinoids, carbohydrates, chlorophylls, enzymes, co-enzymes, fatty acids, lipids, minerals, nucleic acids, pigments, proteins, or vitamins.

11. A system for releasing a cellular component of a photosynthetic organism, comprising:

a bioreactor comprising;

a container having an exterior surface and an interior surface, the interior surface defining an isolated space configured to retain a plurality of photosynthetic organisms and cultivation media, and

a first lighting system comprising one or more energy-emitting substrates received in the isolated space of the container, each having a first surface and a second surface opposite to the first surface, the one or more energy-emitting substrates configured to supply a first amount of energy from the first surface and a second amount of energy from the second surface to at least some of a plurality of photosynthetic organisms retained in the isolated space; and

cultivation media for sustaining a plurality of photosynthetic organisms, the cultivation media comprising at least one photosensitizer;

wherein the first lighting system is operable to selectively emit energy having a peak emission wavelength ranging from about 400 nm to about 780 nm during a first period of time, and operable to selectively emit energy having a peak emission wavelength ranging from about 200 nm to about 400 nm during a second period of time, different than the first.

12. The system of claim 11 wherein the second amount of energy has at least one characteristic that has a value that is different than a value of a characteristic of the first amount of energy.

13. The system of claim 11 wherein the at least one characteristic is at least one of a light intensity, an illumination intensity, an energy-emitting pattern, a peak emission wavelength, an on-pulse duration, and/or a pulse frequency.

14. The system of claim 11 wherein the photosensitizer is energy activatable, and the first lighting system is operable to selectively emit energy having a peak emission wavelength corresponding to an activation energy of the photosensitizer.

15. The system of claim 11 wherein the one or more energy-emitting substrates are configured to supply an effective amount of energy to a substantial portion of the plurality of photosynthetic organisms retained in the isolated space, the effective amount of energy sufficient to cause the at least one photosensitizer to disrupt one or more membrane structures, tubules, vesicles, cisternae, organelles, cell compartments, plastids, or mitochondria, associated with the plurality of photosynthetic organisms.

16. A composition for releasing one or more growth factors, amino acids, nucleic acids, carotenoids, bioflavinoids, carbohydrates, chlorophylls, enzymes and co-enzymes, fatty acids, lipids, minerals, nucleic acids, pigments, proteins, and/or vitamins from an algal biomass into a collection medium, the composition comprising:

a plurality of energy-activatable photosensitizers, the energy-activatable photosensitizers activatable by absorption of light, sonic, ultrasonic, thermal, and/or chemical energy;

a permeabilizer to promote absorption of the energy-activatable photosensitizers by the algal biomass.

17. The composition of claim 16 wherein the algal biomass is selected from a group comprising prokaryotic algae and eukaryotic algae.

18. The composition of claim 16 wherein the algal biomass is selected from one or more micro-algae.

19. A process for producing and recovering one or more cell components from culture media including a plurality of photosynthetic organisms, comprising:

inducing a dielectric change in the culture media, the induced dielectric change sufficient to induce photo-oxidative stress of a substantial portion of the plurality of photosynthetic organisms; and

recovering the cultivation media comprising the one or more cell components.

20. The process of claim 19 wherein recovering the cultivation media comprising the one or more cell components includes chromatographically recovering one or more growth factors, amino acids, carotenoids, bioflavinoids, carbohydrates, chlorophylls, enzymes and co-enzymes, fatty acids, lipids, minerals, nucleic acids, pigments, proteins, and vitamins.

21. The process of claim 19, wherein the plurality of photosynthetic organisms is selected from a group comprising prokaryotic algae and eukaryotic algae.

22. The process of claim 19, wherein the plurality of photosynthetic organisms is selected from one or more micro-algae.