SOLUTIONS FOR TISSUE ENGINEERING AND METHODS OF USE
First Claim
1. A method of preparing a decellularized tissue for recellularization comprising the steps of conditioning the collagen in said decellularized tissue and biologically preparing said decellularized tissue by placing said decellularized tissue in an environment that promotes the recellularization of said tissue.
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Abstract
The present invention provides for solutions and methods of preparing a decellularized tissue for recellularization. The solutions provide collagen conditioning to restore collagen triple helix structure, strengthening of the collagen structure of the tissue, and biologically preparing the decellularized tissue by placing it in an environment that promotes recellularization. Methods and solutions for recellularization of a decellularized tissue, in accordance with the present invention, are also provided.
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Citations
36 Claims
- 1. A method of preparing a decellularized tissue for recellularization comprising the steps of conditioning the collagen in said decellularized tissue and biologically preparing said decellularized tissue by placing said decellularized tissue in an environment that promotes the recellularization of said tissue.
- 13. A solution for the treatment of tissue comprising ethyl alcohol, lauryl alcohol, myristyl alcohol, and hyaluronan HMW salt.
- 16. A solution for the treatment of tissue comprising one or more solvent alcohols, one or more fatty alcohols, one or more lubricants, one or more ECM protein restoration agents, and combinations thereof.
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17. A solution for the treatment of tissue comprising:
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(a) A serum solution comprising serum, cell culture medium, insulin, and buffer; (b) A growth factor solution comprising one or more growth factors and at least one myofibroblast phenotype maintenance agent; (c) A nutrient solution to accelerate cell seeding comprising steroid-like compounds, a collagen cross-linking agent; and (d) At least one antibiotic. - View Dependent Claims (12, 18, 19, 20, 21, 29, 30)
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24. A method of reseeding a decellularized tissue comprising the steps of
(a) A seeding phase; - and
(b) A cell differentiation and migration phase. - View Dependent Claims (25, 26, 27, 28, 31, 32, 33, 34, 35, 36)
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Specification