Biological Analysis Systems, Devices, and Methods

• US 20100035358A1
• Filed: 10/07/2009
• Published: 02/11/2010
• Est. Priority Date: 10/05/2007
• Status: Abandoned Application

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Lehto, Dennis A., Boege, Steven J.
• Application Number:
  US12/575,004
• Publication Number:
  US 20100035358A1
• Time in Patent Office: Days
• US Class Current: 436/164
• CPC Class Codes: B01J 2219/00286 Reactor vessels with top and bottom openings
• CPC Class Codes: B01J 2219/00306 Reactor vessels in a multiple arrangement
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/00533 essentially rectangular
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00648 by the use of solid beads
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2300/043 Hinged closures
• CPC Class Codes: B01L 2300/0822 Slides
• CPC Class Codes: B01L 2300/0877 Flow chambers
• CPC Class Codes: B01L 2300/1805 Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
• CPC Class Codes: B01L 2300/1811 using electromagnetic induction heating
• CPC Class Codes: B01L 2300/1894 Cooling means; Cryo cooling
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: B01L 3/50851 specially adapted for heating or cooling samples laboratory heating apparatus B01L7/00; incubators C12M
• CPC Class Codes: B01L 7/00 Heating or cooling apparatus autoclaves B01J3/04; Heat insulating devices
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: C12Q 1/6874 involving nucleic acid arrays, e.g. sequencing by hybridisation
• CPC Class Codes: G01N 15/1433 using image recognition
• CPC Class Codes: G01N 15/1459 : the analysis being performed on a sample stream
• CPC Class Codes: G01N 15/1484 : microstructural devices
• CPC Class Codes: G01N 2021/0325 : Cells for testing reactions, e.g. containing reagents
• CPC Class Codes: G01N 2021/0346 : Capillary cells; Microcells
• CPC Class Codes: G01N 2021/058 : Flat flow cell
• CPC Class Codes: G01N 21/0332 : with temperature control control of temperature G05D23/00; cryostats F17C3/08
• CPC Class Codes: G01N 21/05 : Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 2201/02 : Mechanical
• CPC Class Codes: G03B 27/42 : for automatic sequential copying of the same original G03B27/34, G03B27/53 take precedence

Abstract

A device for performing biological sample reactions may include a plurality of flow cells configured to be mounted to a common microscope translation stage, wherein each flow cell is configured to receive at least one sample holder containing biological sample. Each flow cell also may be configured to be selectively placed in an open position for positioning the at least one sample holder into the flow cell and a closed position for reacting biological sample contained in the at least one sample holder. The plurality of flow cells may be configured to be selectively placed in the open position and the closed position independently of each other.

Claims

1. A device for performing biological sample reactions, the device comprising:

a plurality of flow cells configured to be removably mounted relative to a microscope translation stage;

wherein each of the plurality of flow cells is configured to receive a sample holder containing at least one biological sample,

wherein each of the plurality of flow cells is further configured to receive fluid when mounted relative to the microscope translation stage to enable a biochemical reaction of the at least one biological sample.

2. The device of claim 1, further comprising a frame mounted to the microscope translation stage, wherein the plurality of flow cells are configured to be removably mounted to the frame.

3. The device of claim 1, further comprising a thermal system configured to control a temperature of the at least one biological sample received by the plurality of flow cells.

4. The device of claim 1, wherein the thermal system comprises at least one forced air cooling component.

5. The device of claim 1, wherein the thermal system comprises at least one thermoelectric component.

6. The device of claim 1, wherein each of the plurality of flow cells is configured to be mounted relative to a benchtop fixture to receive the sample holder.

7. The device of claim 1, wherein each of the plurality of flow cell is configured to receive a substrate comprising an array of microparticles comprising nucleic acids.

8. The device of claim 1, wherein the device is configured to perform nucleic acid sequencing.

9. The device of claim 1, wherein each of the sample holders received by the plurality of flow cells is configured to be imaged when the plurality of flow cells are mounted relative to the microscope translation stage.

10. The device of claim 1, wherein the plurality of flow cells are configured to permit a biochemical reaction of sample to occur in at least one of the plurality of plurality of flow cells with a substrate comprising an array of microparticles comprising nucleic acid templates.

11. A method for performing biological analysis, the method comprising:

respectively providing a plurality of flow cells with a sample holder containing at least one sample for biological analysis;

mounting each of the plurality of flow cells relative to a microscope translation stage; and

performing a biochemical reaction in each of the plurality of flow cells while the plurality of flow cells are mounted relative to the microscope translation stage,

wherein the respectively providing the plurality of flow cells with a sample holder occurs prior to the mounting of the plurality of flow cells relative to the microscope translation stage.

12. The method of claim 11, wherein the respectively providing the plurality of flow cells with a sample holder occurs while the plurality of flow cells are mounted relative to a benchtop fixture.

13. The method of claim 11, wherein the respectively providing the plurality of flow cells with a sample holder comprises respectively providing each of the flow cells while analysis of a reacted sample occurs in at least one other of the plurality of flow cells.

14. The method of claim 11, further comprising imaging the sample holder received by the plurality of flow cells.

15. The method of claim 11, wherein the performing the biochemical reaction comprises sequencing nucleic acids.

16. The method of claim 11, further comprising performing a biochemical reaction in at least one of the plurality of flow cells while analyzing a reaction of the sample that has occurred in at least one other of the plurality of flow cells while the plurality of flow cells are mounted relative to the microscope translation stage.

17. The method of claim 11, further comprising controlling a temperature of the at least one sample.

18. The method of claim 17, wherein controlling the temperature comprises forcing air proximate the plurality of flow cells.

19. The method of claim 17, wherein controlling the temperature comprises controlling the temperature via a thermoelectric component.