METHOD FOR ISOLATION OF AFTERBIRTH DERIVED CELLS
First Claim
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1. A method for extracting cells from afterbirth tissue, comprising the steps of:
- a) locating the afterbirth tissue in a perfusion circuit;
b) extracting cells from said afterbirth tissue by pulsatile perfusion using a perfusion solution to produce a perfusate containing cells; and
d) isolating cells from the perfusate.
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Abstract
A method for extracting cells from afterbirth tissue, including placing the afterbirth into a perfusion circuit prior to exsanguination, extracting the cells from the afterbirth with digestive enzymes and mechanically recovering cells from the digested afterbirth tissue, and isolating the cells from the perfusate and digestion mix. Also disclosed is a cell line derived from afterbirth using the two-step pulsatile perfusion extraction method.
31 Citations
19 Claims
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1. A method for extracting cells from afterbirth tissue, comprising the steps of:
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a) locating the afterbirth tissue in a perfusion circuit; b) extracting cells from said afterbirth tissue by pulsatile perfusion using a perfusion solution to produce a perfusate containing cells; and d) isolating cells from the perfusate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification