Raman Instrument for Measuring Weak Signals in the Presence of Strong Background Fluorescence

• US 20100042001A1
• Filed: 06/12/2009
• Published: 02/18/2010
• Est. Priority Date: 10/04/2005
• Status: Active Grant

• Current Assignee: NSE Products, Inc. (Nu Skin Enterprises Incorporated)
• Original Assignee: NSE Products, Inc. (Nu Skin Enterprises Incorporated)
• Inventors: Ferguson, Scott, Fralick, John F., Bergeson, Scott Douglas, Peatross, Justin
• Granted Patent: US 8,160,681 B2
• Time in Patent Office: Days
• US Class Current: 600/476
• CPC Class Codes: A61B 2560/0223 of calibration, e.g. protocols for calibrating sensors
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: G01J 3/02 Details
• CPC Class Codes: G01J 3/0291 Housings; Spectrometer accessories; Spatial arrangement of elements, e.g. folded path arrangements
• CPC Class Codes: G01J 3/44 Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
• CPC Class Codes: G01N 2021/6419 Excitation at two or more wavelengths
• CPC Class Codes: G01N 2021/6421 Measuring at two or more wavelengths
• CPC Class Codes: G01N 21/274 Calibration, base line adjustment, drift correction
• CPC Class Codes: G01N 21/65 Raman scattering

Abstract

A method for measuring a chemical concentration in tissue has two measurement steps. A portion of tissue is illuminated with a first generated light and a second generated light. A first Raman scattered light, corresponding to the first generated light, and a second Raman scattered light, corresponding to the second generated light, are directed to a plurality of light sensors, each light sensor measuring light at a different wavelength, that wavelength being proximate to a wavelength of an expected Raman shift wavelength for the chemical in the tissue. A measurement is obtained from each light sensor, each measurement being specific to the first scattered light and/or the second reflected light to that light sensor. The measurements of the first scattered light and the measurements of the second scattered light are used to calculate a concentration of the chemical in the tissue.

Claims

1-20. -20. (canceled)

21. An apparatus for measuring the level of a selected molecule in a target sample, comprising:

means for illuminating the target with light of a first wavelength F1;

means for illuminating the target with light of a second wavelength F2 adjacent to the first wavelength F1;

means for receiving from the target emitted light resulting from each of the illuminations at wavelengths F1 and F2 and sampling the intensity of the emitted light at a range of wavelengths including a characteristic Raman emission wavelength Fc associated with the selected molecule and each of the illumination wavelengths;

means for developing a first set of sampled intensity values for emitted light resulting from the illumination for wavelength F1, said sample including a value at or near the characteristic emission wavelength Fc associated with the selected molecule and F1, at least one sample adjacent the characteristic wavelength Fc but sufficiently displaced below that wavelength to exclude Raman emissions resulting from the illumination for wavelength F1 and at least one sample adjacent the characteristic wavelength Fc but sufficiently displaced above that wavelength to exclude Raman emissions resulting from the illumination for wavelength F1;

means for developing a second set of sampled intensity values for emitted light resulting from the illumination for wavelength F2, said sample including a value at or near the characteristic emission wavelength Fc associated with the selected molecule and F2, at least one sample adjacent the characteristic wavelength Fc but sufficiently displaced below that wavelength to exclude Raman emissions resulting from the illumination for wavelength F2 and at least one sample adjacent the characteristic wavelength Fc but sufficiently displaced above that wavelength to exclude Raman emissions resulting from the illumination for wavelength F2; and

means for deriving from ratios of corresponding values in the first and second sets of sampled intensity values an interpolated intensity value for emitted light between the characteristic wavelength Fc associated with each of F1 and F2 that removes the intensity value component due to non-Raman emissions.

22. The apparatus of claim 21 wherein the said means for developing a first and second set of sampled intensity values for emitted light resulting from the illumination for wavelength F1 and the wavelength F2, respectively, comprises comprise at least four intensity sensors with data processing logic to sense four samples value sample values resulting from the illumination for wavelength F1 {SF10, SF11, SF12, SF13} and data processing logic to sense four sample values resulting from the illumination at wavelength F2 {SF20, SF21, SF22, SF23}, where each of {SF10, SF11, SF12, SF13} and {SF20, SF21, SF22, SF23 } is an ordered set of values with SF11 being a measurement at characteristic emitted wavelength Fc associated with the selected molecule and F1, and SF22 being a measurement at characteristic emitted wavelength Fc associated with the selected molecule and F2.

23. The apparatus of claim 22 further comprising means for adjusting each of {SF10, SF11, SF12, SF13} and {SF20, SF21, SF22, SF23} for variations in the output of the at least four intensity sensors since a calibration of such sensors.

24. The apparatus of claim 22 further comprising means for adjusting each of {SF10, SF1, SF12, SF13} and {SF20, SF21, SF22, SF23} for a dark value derived from an optically dark sample target with no Raman scattering in the light emitted from the sample.

25. The apparatus of claim 21 wherein the said means for deriving from ratios of corresponding values in the first and second sets of sampled intensity values an interpolated intensity value comprises data processing logic that computes the sample values ratios {SF10/SF20, SF11/SF21, SF12/SF22, SF13/SF23} and from resulting computed sample values ratios, interpolates by curve fitting to find a curve that passes through points {f0, SF10/SF20} and {f3, SF13/SF23} on a wavelength-ratio graph and the curve is equidistant from points {f1, SF11/SF21} and {f2, SF12/SF22} on the graph, where each of f0, f1, f2 and f3 is a center wavelength for a respective first, second, third and fourth sensor for a sampled intensity value.

26. The apparatus of claim 25 wherein the said data processing logic for curve fitting uses a parabola as the curve.

27. The apparatus of claim 25, comprising data processing logic using the resulting computed sample value ratios to graphically determine in a parabolic interpolation based on a curve anchored at {f0, SF10/SF20} and {f3, SF13/SF23} a value that is at the midpoint of the wavelength range for the four sensors and that lies midway between the points {f1, SF11/SF21] and {f2, SF12/SF22}.

28. The apparatus of claim 21, comprising data processing logic that computes sample value ratios {SF10/SF20, SF11/SF21, SF12/SF22, SF13/SF23} and uses the resulting computed sample value ratios to graphically determine a value that lies midway between the points {f1, SF11/SF21} and {f2, SF12/SF22} on a wavelength-ratio graph as measured by curve fitting with a non-linear curve anchored at {f0, SF10/SF20} and {f3, SF13/SF23} on the graph.

29. The apparatus of claim 21 wherein the means for receiving from the target emitted light resulting from each of the illuminations at wavelength F1 and F2 and sampling the intensity of the emitted light at a range of wavelengths including a characteristic Raman emitted wavelength Fc associated with the selected molecule and each of the illumination wavelengths comprises means for directing the emitted light in predefined portions to at least four intensity sensors.

30. The apparatus of claim 29 wherein the means for directing the emitted light in predefined portions to at least four intensity sensors comprises means for directing the emitted light in equal portions.

31-43. -43. (canceled)

44. An apparatus for measuring the level of a selected molecule in a target sample, comprising:

a first light source that illuminates the target with light of a first wavelength F1;

a second light source that illuminates the target with light of a second wavelength F2 adjacent to the first wavelength F1;

means for receiving from the target emitted light resulting from each of the illuminations at wavelengths F1 and F2 and sampling the intensity of the emitted light at a range of wavelengths including a characteristic Raman emission wavelength Fc associated with the selected molecule and each of the illumination wavelengths;

a first sampler that develops a first set of sampled intensity values for emitted light resulting from the illumination for wavelength F1, said sample including a value at or near the characteristic emission wavelength Fc associated with the selected molecule and F1, at least one sample adjacent the characteristic wavelength Fc but sufficiently displaced below that wavelength to exclude Raman emissions resulting from the illumination for wavelength F1 and at least one sample adjacent the characteristic wavelength Fc but sufficiently displaced above that wavelength to exclude Raman emissions resulting from the illumination for wavelength F1;

a second sampler that develops a second set of sampled intensity values for emitted light resulting from the illumination for wavelength F2, said sample including a value at or near the characteristic emission wavelength Fc associated with the selected molecule and F2, at least one sample adjacent the characteristic wavelength Fc but sufficiently displaced below that wavelength to exclude Raman emissions resulting from the illumination for wavelength F2 and at least one sample adjacent the characteristic wavelength Fc but sufficiently displaced above that wavelength to exclude Raman emissions resulting from the illumination for wavelength F2; and

data processing logic that derives from ratios of corresponding values in the first and second sets of sampled intensity values an interpolated intensity value for emitted light between the characteristic wavelength Fc associated with each of F1 and F2 that removes the intensity value component due to non-Raman emissions.

45. The apparatus of claim 44 wherein the sampler that develops a first and second set of sampled intensity values for emitted light resulting from the illumination for wavelength F1 and the wavelength F2, respectively, comprises at least four intensity sensors with data processing logic to sense four sample values resulting from the illumination for wavelength F1 {SF10, SF11, SF12, SF13} and data processing logic to sense four sample values resulting from the illumination at wavelength F2 {SF20, SF21, SF22, SF23}, where each of {SF10, SF11, SF12, SF13} and {SF20, SF21, SF22, SF23} is an ordered set of values with SF11 being a measurement at characteristic emitted wavelength Fc associated with the selected molecule and F1, and SF22 being a measurement at characteristic emitted wavelength Fc associated with the selected molecule and F2.

46. The apparatus of claim 45 further comprising means for adjusting each of {SF10, SF11, SF12, SF13} and {SF20, SF21, SF22, SF23} for variations in the output of the at least four intensity sensors since a calibration of such sensors.

47. The apparatus of claim 45 further comprising means for adjusting each of {SF10, SF11, SF12, SF13} and {SF20, SF21, SF22, SF23} for a dark value derived from an optically dark sample target with no Raman scattering in the light emitted from the sample.

48. The apparatus of claim 44 wherein the data processing logic that derives from ratios of corresponding values in the first and second sets of sampled intensity values an interpolated intensity value comprises curve fit data processing logic that computes sample values ratios {SF10/SF20, SF11/SF21, SF12/SF22, SF13/SF23} and from resulting computed sample values ratios, interpolates by curve fitting to find a curve that passes through points {f0, SF10/SF20} and {f3, SF13/SF23} on a wavelength-ratio graph and the curve is equidistant from points {f1, SF11/SF21} and {f2, SF12/SF22} on the graph, where each of f0, f1, f2 and f3 is a center wavelength for a respective first, second, third and fourth sensor for a sampled intensity value.

49. The apparatus of claim 48 wherein the said data processing logic for curve fitting uses a parabola as the curve.

50. The apparatus of claim 48, comprising data processing logic using the resulting computed sample value ratios to graphically determine in a parabolic interpolation based on a curve anchored at {f0, SF10/SF20} and {f3, SF13/SF23} a value that is at the midpoint of the wavelength range for the four sensors and that lies midway between the points {f1, SF11/SF21] and {f2, SF12/SF22}.

51. The apparatus of claim 44, comprising data processing logic that computes sample value ratios {SF10/SF20, SF11/SF21, SF12/SF22, SF13/SF23} and using resulting computed sample value ratios to graphically determine a value that lies midway between points {f1, SF11/SF21} and {f2, SF12/SF22} on a wavelength-ratio graph as measured by curve fitting with a non-linear curve anchored at {f0, SF10/SF20} and {f3, SF13/SF23} on the graph.

52. The apparatus of claim 44 wherein the means for receiving from the target emitted light resulting from each of the illuminations at wavelength F1 and F2 and sampling the intensity of the emitted light at a range of wavelengths including a characteristic Raman emitted wavelength Fc associated with the selected molecule and each of the illumination wavelengths comprises means for directing the emitted light in predefined portions to at least four intensity sensors.

53. The apparatus of claim 52 wherein the means for directing the emitted light in predefined portions to at least four intensity sensors comprises means for directing the emitted light in equal portions.