The Method for Enhancement of Photosynthesis and Biomass of Plant by Plastid Transformation of Malate Dehydrogenase
First Claim
1. A transgenic plant prepared by inserting MDH (malate dehydrogenase) gene originated from a bacteria into the plastid genome of a target plant.
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Abstract
The present invention relates to a method for enhancement of photosynthesis and biomass of a plant by plastid transformation with MDH gene, more precisely a method for enhancement of photosynthesis and biomass of C3 plant by plastid transformation system with MDH gene. The MDH plastid transgenic plant prepared by the method of the present invention exhibits not only increased photosynthesis efficiency but also increased growth rate, leaf area, stem diameter and biomass of the plant, compared with the control plant. Therefore, the plastid transformed C3 plant prepared by C4 type gene introduction can be effectively used for enhancing photosynthesis and biomass of the plant.
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Citations
17 Claims
- 1. A transgenic plant prepared by inserting MDH (malate dehydrogenase) gene originated from a bacteria into the plastid genome of a target plant.
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6. A method for preparing a transgenic plant with the insertion of MDH gene originated from a bacteria in its plastid genome, comprising the following steps:
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1) constructing a vector for MDH plastid transformation by inserting MDH (malate dehydrogenase) gene sequence which is active in plastid into a vector for plastid transformation; 2) preparing a transformant by introducing the vector for MDH plastid transformation into C3 plant or C3 plant culture cell; 3) culturing the transformant of step
2); and4) re-differentiating the cultured transformant of step
3) by tissue culture. - View Dependent Claims (7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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Specification